Anti-PPAR gamma Rabbit Monoclonal Antibody

Western blot analysis of PPAR gamma expression in HeLa cell lysate.

H19 and PPARγ are upregulated in the femoral head and BMSCs of patients with SONFH. (A, B) X-ray photo and pathological structure of the femoral head from an ARCO stage V SONFH patient. The images show alterations in the morphology of the femoral head, characterized by collapse and flattening, as well as radiographic signs indicative of hip osteoarthritis. (C) Morphology of BMSCs from a patient with SONFH. (D, E) Expression levels of H19 and PPARγ in the femoral head and BMSCs from a patient with SONFH. All experimental procedures were performed in triplicate with internal normalization to GAPDH expression levels. The relative expression levels of each gene were analyzed using the 2 −△△Ct method (n = 8, all data are shown as the mean ± SD of three independent experiments, *p < 0.05, **p < 0.01).
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H19 participates in increased adipogenesis of BMSCs and positively regulates PPARγ expression in SONFH. (A) Expression level of H19 in BMSCs after transfection with sh-H19. (B) Oil Red O staining (200×) of BMSCs after transfection with sh-H19. (C, D) Quantification of Oil Red O staining (200×) of BMSCs after transfection with sh-H19. (E, F) The expression of fatty acid-binding protein 4 (FABP4) was detected by qRT-PCR and Western blot after knocking-down of H19. (G) The expression levels of H19 and PPARγ 1 week following the knockdown of H19. (n = 3, all data are shown as the mean ± SD of three independent experiments, *p < 0.05, **p < 0.01).
Index in PubMed under a CC BY license. PMID: 40259926

H19 modulates PPARγ expression through miR-130b-3p. (A) Three databases, StarBase, DIANA tools, and miRWalk, were used to predict candidate micro (mi)RNAs as shown in the Venn diagram. (B) Expression levels of three candidate miRNAs (miR-301b-3p, miR-130b-3p, and miR-130a-3p) after knockdown of H19. (C) The binding sites of miR-130b-3p with H19 and PPARγ. (D) Effect of miR-130b-3p on the luciferase activity of wild-type (WT)-H19 and mutant (MT)-H19 reporter systems. (E) Effect of miR-130b-3p on the luciferase activity of WT-FABP4 and MT-FABP4 reporter systems was detected via luciferase reporter assay. (F, G) The expression of PPARγ is significantly decreased upon knocking down H19. This reduction could be reversed through co-transfection with a miR-130b-3p inhibitor. (H, I) The expression of PPARγ was significantly augmented when H19 was upregulated. This elevation in PPARγ expression could be counteracted by co-transfecting with a miR-130b-3p mimic. (n = 3, all data are shown as the mean ± SD of three independent experiments, **p < 0.01, ***p < 0.001).
Index in PubMed under a CC BY license. PMID: 40259926

All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.