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Product Info Summary
| SKU: | A07755 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PPP1R3A Antibody
SKU/Catalog Number
A07755
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PPP1R3A Antibody (Catalog# A07755). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPP1R3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07755)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PPP1R3A. Immunogen sequence location: 647 - 696
Reactive Species
A07755 is reactive to PPP1R3A in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
125.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07755 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of the lysates from HT-29 cells using PPP1R3A antibody.
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Western blot analysis of lysates from MCF-7 and COLO cells, using PPP1R3A Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-PPP1R3A Antibody (A07755)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-PPP1R3A Antibody
Question
Would anti-PPP1R3A antibody A07755 work on pig WB with skeletal muscle?
C. Collins
Verified customer
Asked: 2020-04-16
Answer
Our lab technicians have not validated anti-PPP1R3A antibody A07755 on pig. You can run a BLAST between pig and the immunogen sequence of anti-PPP1R3A antibody A07755 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig skeletal muscle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-16
Question
Do you have a BSA free version of anti-PPP1R3A antibody A07755 available?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PPP1R3A antibody A07755 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-08
Question
We are currently using anti-PPP1R3A antibody A07755 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
The anti-PPP1R3A antibody (A07755) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-14
Question
Is this A07755 anti-PPP1R3A antibody reactive to the isotypes of PPP1R3A?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
The immunogen of A07755 anti-PPP1R3A antibody is A peptide derived from human PPP1R3A. Immunogen sequence location: 647 - 696. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-27
Question
Would A07755 anti-PPP1R3A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Huang
Verified customer
Asked: 2018-11-13
Answer
You can see on the product datasheet, A07755 anti-PPP1R3A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-13
