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Product Info Summary
| SKU: | PB9730 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Oligodendrocyte myelin glycoprotein/OMG Antibody Picoband®
SKU/Catalog Number
PB9730
PB0771 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Oligodendrocyte myelin glycoprotein/OMG Antibody Picoband® catalog # PB9730. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Oligodendrocyte myelin glycoprotein/OMG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9730)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human OMG, different from the related mouse sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9730 is reactive to OMG in Human, Mouse, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
49.6 kDa
Background of OMG
Oligodendrocyte-myelin glycoprotein is a protein that in humans is encoded by the OMG gene. This gene is mapped to 17q11-q12. OMG, like neurofibromin (NF1), has growth suppressive effects and downregulates mitogenic signaling pathways closely related to those influenced by neurofibromin. Overexpression of OMG alters mitogenic signaling in fibroblasts. Cells overexpressing OMG grow more slowly in serum compared to controls and show a partial G1 block upon cell cycle analysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9730 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, U87 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of OMG using anti-OMG antibody (PB9730).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug,
Lane 3: U87 Whole Cell Lysate at 40ug.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OMG antigen affinity purified polyclonal antibody (Catalog # PB9730) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OMG at approximately 50KD. The expected band size for OMG is at 50KD.
Specific Publications For Anti-Oligodendrocyte myelin glycoprotein/OMG Antibody Picoband® (PB9730)
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7 Customer Q&As for Anti-Oligodendrocyte myelin glycoprotein/OMG Antibody Picoband®
Question
Please see the WB image, lot number and protocol we used for bronchial epithelial cell using anti-Oligodendrocyte myelin glycoprotein/OMG antibody PB9730. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-20
Question
I see that the anti-Oligodendrocyte myelin glycoprotein/OMG antibody PB9730 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bronchial epithelial cell using anti-Oligodendrocyte myelin glycoprotein/OMG antibody PB9730. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-26
Question
Is a blocking peptide available for product anti-Oligodendrocyte myelin glycoprotein/OMG antibody (PB9730)?
Verified Customer
Verified customer
Asked: 2018-10-18
Answer
We do provide the blocking peptide for product anti-Oligodendrocyte myelin glycoprotein/OMG antibody (PB9730). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-10-18
Question
We are currently using anti-Oligodendrocyte myelin glycoprotein/OMG antibody PB9730 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-20
Answer
The anti-Oligodendrocyte myelin glycoprotein/OMG antibody (PB9730) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-20
Question
Will PB9730 anti-Oligodendrocyte myelin glycoprotein/OMG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-10
Answer
As indicated on the product datasheet, PB9730 anti-Oligodendrocyte myelin glycoprotein/OMG antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-10
Question
Can you help my question with product PB9730, anti-Oligodendrocyte myelin glycoprotein/OMG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9730 anti-Oligodendrocyte myelin glycoprotein/OMG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-28
