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Product Info Summary
| SKU: | M08947 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-Prominin 2 (PROM2) Mouse Monoclonal Antibody [Clone ID: OTI3D12]
SKU/Catalog Number
M08947
Size
100 µl
Description
Boster Bio Anti-PROM2 (Prominin 2) mouse monoclonal antibody, clone OTI3D12 (formerly 3D12). Catalog# M08947. Tested in IF, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Prominin 2 (PROM2) Mouse Monoclonal Antibody [Clone ID: OTI3D12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08947)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3D12
Isotype
IgG2b
Immunogen
Full-length protein expressed in 293T cell transfected with human PROM2 expression vector
Reactive Species
M08947 is reactive to PROM2 in Human
Calculated molecular weight
91.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08947 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IF 1:100
Validation Images & Assay Conditions
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Western blot analysis of 35ug of cell extracts from canine Kidney (MDCK) cells using anti-PROM2 antibody.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROM2.
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Western blot analysis of 35ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-PROM2 antibody.
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Western blot analysis of 35ug of cell extracts from human (HeLa) cells using anti-PROM2 antibody.
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Western blot analysis of 35ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-PROM2 antibody.
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Western blot analysis of 35ug of cell extracts from human Lung adenocarcinoma (A549) cells using anti-PROM2 antibody.
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Immunofluorescent staining of Cos7 cells using anti-PROM2 mouse monoclonal antibody (M08947).
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Immunofluorescent staining of A549 cells using anti-PROM2 mouse monoclonal antibody (M08947).
Specific Publications For Anti-Prominin 2 (PROM2) Mouse Monoclonal Antibody [Clone ID: OTI3D12] (M08947)
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