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Product Info Summary
| SKU: | M05105 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-Proteasome subunit beta type 4 (PSMB4) Mouse Monoclonal Antibody [Clone ID: OTI5B5]
SKU/Catalog Number
M05105
Size
100 µl
Description
Boster Bio PSMB4 (Proteasome subunit beta type 4 ) mouse monoclonal antibody, clone OTI5B5 (formerly 5B5). Catalog# M05105. Tested in FC, IF, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Proteasome subunit beta type 4 (PSMB4) Mouse Monoclonal Antibody [Clone ID: OTI5B5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05105)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5B5
Isotype
IgG1
Immunogen
Full length human recombinant protein of human PSMB4 (NP_002787) produced in HEK293T cell.
Reactive Species
M05105 is reactive to PSMB4 in Human, Mouse, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
29.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05105 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells transfected with either PSMB4 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PSMB4 antibody (M05105)
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Anti-PSMB4 mouse monoclonal antibody (M05105) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMB4.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB4.
Specific Publications For Anti-Proteasome subunit beta type 4 (PSMB4) Mouse Monoclonal Antibody [Clone ID: OTI5B5] (M05105)
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