Product Info Summary
SKU: | A00380 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Protein S100-A9 S100A9 Antibody
SKU/Catalog Number
A00380
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Protein S100-A9 S100A9 Antibody catalog # A00380. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein S100-A9 S100A9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00380)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Survivin around the phosphorylation site of T34.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with eNOS or nNOS.
Reactive Species
A00380 is reactive to S100A9 in Human
Applications
A00380 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
13.242kDa
Background of S100A9
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
David A. Jans, J. Biol. Chem., Nov 1996; 271: 30781.
Eugene Estella, Diabetes, Aug 2006; 55: 2212 - 2219.
Yong Gu, J. Biol. Chem., May 1996; 271: 10816.
Jiuru Sun, J. Biol. Chem., Nov 1996; 271: 27802.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunocytochemistry staining of S100A9 using Anti-Protein S100-A9 S100A9 Antibody (A00380).
ICC staining S100A9 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 2. Immunocytochemistry staining of S100A9 using Anti-Protein S100-A9 S100A9 Antibody (A00380).
ICC staining S100A9 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeProtein Target Info & Infographic
Gene/Protein Information For S100A9 (Source: Uniprot.org, NCBI)
Gene Name
S100A9
Full Name
Protein S100-A9
Weight
13.242kDa
Superfamily
S-100 family
Alternative Names
CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; Human S100A9 Capture; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9 S100A9 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14 S100 calcium binding protein A9 protein S100-A9|MRP-14|calgranulin B|calprotectin L1H subunit|leukocyte L1 complex heavy chain|migration inhibitory factor-related protein 14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on S100A9, check out the S100A9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for S100A9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein S100-A9 S100A9 Antibody (A00380)
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No publications found for A00380
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