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- Table of Contents
PROX1 antibody (A01985-1) performs well in ELISA, Flow Cytometry, IP, IF, ICC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of SH-SY5Y cells using anti-PROX1 antibody (A01985-1).
Overlay histogram showing SH-SY5Y cells stained with A01985-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROX1 Antibody (A01985-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SH-SY5Y cells stained with A01985-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROX1 Antibody (A01985-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PROX1 Antibody Picoband®
PROX1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | ELISA, Flow Cytometry, IP, IF, ICC, WB |
Tested Applications
PROX1 antibody was positively detected by WB in:
human HepG2 whole cell, human HUH7 whole cell, human SH-SY5Y whole cell, rat RH35 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PROX1 antibody was positively detected by IF in:
SiHa cell
PROX1 antibody was positively detected by IP in:
SH-SY5Y cell
PROX1 antibody was positively detected by FC in:
SH-SY5Y cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ICC
ELISA
Human
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PROX1 Antibody Picoband®
View all PROX1 Antibodies
View all PROX1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PROX1 recombinant protein (Position: E51-E737).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
90 kDa
Calculated Molecular Weight
83.2 kDa
Background of PROX1
Prospero homeobox protein 1, also called PROX1 is a protein that in humans is encoded by the PROX1 gene. This gene is mapped to 1q32.3. The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus.
Cite This Product
Anti-PROX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01985-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
99%
High Homology
Rabbit
99%
High Homology
Mouse
98%
High Homology
Sheep
98%
High Homology
Zebrafish
83%
Moderate Homology
Cow
78%
Moderate Homology
Rat
75%
Moderate Homology
Drosophila
29%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PROX1 antibody
Immunofluorescence (IF) protocol for PROX1 antibody
Immunoprecipitation (IP) protocol for PROX1 antibody
Flow Cytometry (FC) protocol for PROX1 antibody
Publications
Application:
Species:
Prospero-related homeobox protein 1 (Prox1) enhances myelin sheath regeneration in injured sciatic nerve
Published: 2025-09-24
Journal: IBRO Neuroscience Reports
Application: IHC,IF,WB
Impact Factor: 2.90
Species: Mouse
PMID: 41078399
Prox1 induces new lymphatic vessel formation and promotes nerve reconstruction in a mouse model of sciatic nerve crush injury
Published: 2020-06-09
Journal: JOURNAL OF ANATOMY
Application: IHC,IF
Impact Factor: 2.01
Species: Mouse
PMID: 32515838
A novel role of HIF-1α/PROX-1/LYVE-1 axis on tissue regeneration after renal ischaemia/reperfusion in mice
Published: 2018-04-10
Journal: ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
Application: IHC,IF
Impact Factor: 1.81
Species: Mouse
PMID: 29633855
Fucoidan inhibits lymphangiogenesis by downregulating the expression of VEGFR3 and PROX1 in human lymphatic endothelial cells
Published: 2016-05-18
Journal: Oncotarget
Application: WB,IF
Impact Factor: 5.01
Species: Mouse
PMID: 27203545
Review
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Product Promise
We promise Anti-PROX1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01985-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026