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- Table of Contents
CYP2E1 antibody (A00672) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 14 Publications
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Flow Cytometry analysis of SiHa cells using anti-CYP2E1 antibody (A00672).
Overlay histogram showing SiHa cells stained with A00672 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP2E1 Antibody (A00672,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with A00672 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP2E1 Antibody (A00672,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®
CYP2E1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
CYP2E1 antibody was positively detected by WB in:
rat liver tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CYP2E1 antibody was positively detected by IHC in:
human liver cancer tissue, human liver cancer tissue, human liver cancer tissue, mouse liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CYP2E1 antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Human
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®
View all CYP2E1 Antibodies
View all CYP2E1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cytochrome P450 2E1/CYP2E1 recombinant protein (Position: H355-S493).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated Molecular Weight
56.8 kDa
Background of CYP2E1
Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either directly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.
Cite This Product
Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00672)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
93%
High Homology
Rabbit
87%
Moderate Homology
Mouse
86%
Moderate Homology
Rat
86%
Moderate Homology
Cow
86%
Moderate Homology
Dog
84%
Moderate Homology
Sheep
83%
Moderate Homology
Drosophila
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CYP2E1 antibody
Immunohistochemistry (IHC) protocol for CYP2E1 antibody
Flow Cytometry (FC) protocol for CYP2E1 antibody
Publications
Application:
Species:
β-Nicotinamide mononucleotide alleviates Alcohol-Induced liver injury in a mouse model through activation of NAD+/SIRT1 signaling pathways
Published: 2025-08-16
Journal: HEREDITAS
Application: WB
Impact Factor: 2.50
Species: Mouse
PMID: 40819082
Rare Ginsenoside 20(R)-Rg3 Inhibits D-Galactose-Induced Liver and Kidney Injury by Regulating Oxidative Stress-Induced Apoptosis
Published: 2020-07-01
Journal: AMERICAN JOURNAL OF CHINESE MEDICINE
Application: IF
Impact Factor: 3.68
Species: Mouse
PMID: 32668974
Drug-drug interaction of acetaminophen and roxithromycin with the cocktail of cytochrome P450 and hepatotoxicity in rats
Published: 2020-02-04
Journal: International Journal of Medical Sciences
Application: WB
Impact Factor: 2.52
Species: Rat
PMID: 32132876
Hepatoprotective effect of Phellinus linteus mycelia polysaccharide (PL-N1) against acetaminophen-induced liver injury in mouse
Published: 2019-11-20
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 4.78
Species: Mouse
PMID: 31758991
Effect of silibinin on CFLAR-JNK pathway in oleic acid-treated HepG2 cells
Published: 2018-09-21
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 3.46
Species: Human
PMID: 30248539
Dihydrocurcumin ameliorates the lipid accumulation, oxidative stress and insulin resistance in oleic acid-induced L02 and HepG2 cells
Published: 2018-05-07
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 3.46
Species: Human
PMID: 29864915
Improvement of Cisplatin-induced renal dysfunction by Schisandra chinensis stems via anti-inflammation and anti-apoptosis effects
Published: 2018-02-06
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IF
Impact Factor: 3.12
Species: Mouse
PMID: 29421595
Effect of enrofloxacin and roxarsone on CYP450s in pig
Published: 2017-11-21
Journal: RESEARCH IN VETERINARY SCIENCE
Application: WB
Impact Factor: 1.30
Species: Pig
PMID: 29202315
Fructose 1, 6-diphosphate prevents alcohol-induced liver injury through inhibiting oxidative stress and promoting alcohol metabolism in mice
Published: 2017-09-21
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IHC
Impact Factor: 2.90
Species: Mouse
PMID: 28943104
Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis
Published: 2017-07-25
Journal: Journal of Ginseng Research
Application: IF
Impact Factor: 4.08
Species: Mouse
PMID: 30662289
Total sesquiterpene glycosides from Loquat (Eriobotrya japonica) leaf alleviate high-fat diet induced non-alcoholic fatty liver disease through cytochrome P450 2E1 inhibition
Published: 2017-04-28
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: IHC
Impact Factor: 2.76
Species: Mouse
PMID: 28458161
The induction of cytochrome P450 2E1 by ethanol leads to the loss of synaptic proteins via PPARα down-regulation
Published: 2017-04-03
Journal: TOXICOLOGY
Application: ICC
Impact Factor: 3.58
Species: Human
PMID: 28385499
Hepatoprotective Effect of Kaempferol Against Alcoholic Liver Injury in Mice
Published: 2015-04-17
Journal: AMERICAN JOURNAL OF CHINESE MEDICINE
Application: WB
Impact Factor: 2.76
Species: Mouse
PMID: 25787296
Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significanly correlated with cytochrome P450 suppression
Published: 2009-04-21
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: WB
Impact Factor: 2.08
Species: Mouse
PMID: 19370779
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Product Promise
We promise Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00672
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026