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Product Info Summary
| SKU: | A13674 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PRPF18/Prp18 Antibody
SKU/Catalog Number
A13674
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PRPF18/Prp18 Antibody catalog # A13674. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRPF18/Prp18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13674)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PRPF18. AA range:182-231
Reactive Species
A13674 is reactive to PRPF18 in Human, Mouse, Rat
Calculated molecular weight
39.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13674 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot analysis of HuvEc cells using PRPF18 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from 293, MCF-7, and HUVEC cells, using PRPF18 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-PRPF18/Prp18 Antibody (A13674)
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Customer Q&As
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6 Customer Q&As for Anti-PRPF18/Prp18 Antibody
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-PRPF18/Prp18 antibody A13674. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-PRPF18/Prp18 antibody A13674. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-18
Question
Can you help my question with product A13674, anti-PRPF18/Prp18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13674 anti-PRPF18/Prp18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-01
Question
Does anti-PRPF18/Prp18 antibody A13674 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
According to the expression profile of cervix carcinoma, PRPF18 is highly expressed in cervix carcinoma. So, it is likely that anti-PRPF18/Prp18 antibody A13674 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-04-04
Question
We are currently using anti-PRPF18/Prp18 antibody A13674 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
B. Huang
Verified customer
Asked: 2014-03-24
Answer
The anti-PRPF18/Prp18 antibody (A13674) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-24
Question
Is this A13674 anti-PRPF18/Prp18 antibody reactive to the isotypes of PRPF18?
Z. Singh
Verified customer
Asked: 2013-09-26
Answer
The immunogen of A13674 anti-PRPF18/Prp18 antibody is Synthesized peptide derived from the Internal region of human PRPF18. at AA rangle: 160-240. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-09-26
