Anti-PRPF18/Prp18 Antibody

PRP18 antibody

Boster Bio Anti-PRPF18/Prp18 Antibody catalog # A13674. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A13674
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-PRPF18/Prp18 Antibody

View all PRP18 Antibodies

SKU/Catalog Number

A13674

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PRPF18/Prp18 Antibody catalog # A13674. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PRPF18/Prp18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13674)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2H4

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human PRPF18. at AA rangle: 160-240

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A13674 is reactive to PRPF18 in Human, Mouse, Rat

Applications

A13674 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

39.86kDa

Background of PRP18

RNA binding motif protein 47 is a protein in humans that is encoded by the RBM47 gene in chromosome 4. RBM47 may be involved in RNA binding and nucleotide binding.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PRPF18 (Source: Uniprot.org, NCBI)

Gene Name

PRPF18

Full Name

Pre-mRNA-splicing factor 18

Weight

39.86kDa

Superfamily

PRP18 family

Alternative Names

FLJ10210; hPrp18; pre-mRNA-splicing factor 18; PRP18 homolog; PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae); PRP18 pre-mRNA processing factor 18 homolog (yeast); PRP18 pre-mRNA processing factor 18 homolog(PRPF18); PRP18 PRPF18 PRP18, hPrp18 pre-mRNA processing factor 18 pre-mRNA-splicing factor 18|PRP18 homolog|PRP18 pre-mRNA processing factor 18 homolog|PRP18 pre-mRNA processing factor 18 homolog(PRPF18)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRPF18, check out the PRPF18 Infographic

PRPF18 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRPF18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-PRPF18/Prp18 Antibody

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-PRPF18/Prp18 antibody A13674. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-13

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-PRPF18/Prp18 antibody A13674. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-18

Question

Can you help my question with product A13674, anti-PRPF18/Prp18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-06-01

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13674 anti-PRPF18/Prp18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-01

Question

Does anti-PRPF18/Prp18 antibody A13674 work for WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2018-04-04

Answer

According to the expression profile of cervix carcinoma, PRPF18 is highly expressed in cervix carcinoma. So, it is likely that anti-PRPF18/Prp18 antibody A13674 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2018-04-04

Question

We are currently using anti-PRPF18/Prp18 antibody A13674 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

B. Huang

Verified customer

Asked: 2014-03-24

Answer

The anti-PRPF18/Prp18 antibody (A13674) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-03-24

Question

Is this A13674 anti-PRPF18/Prp18 antibody reactive to the isotypes of PRPF18?

Z. Singh

Verified customer

Asked: 2013-09-26

Answer

The immunogen of A13674 anti-PRPF18/Prp18 antibody is Synthesized peptide derived from the Internal region of human PRPF18. at AA rangle: 160-240. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-09-26

Order DetailsPrice
A13674

100uL

$399

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A13674
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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