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Product Info Summary
| SKU: | A15101 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-MRPL52 Antibody
SKU/Catalog Number
A15101
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MRPL52 Antibody (Catalog# A15101). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRPL52 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15101)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MRPL52. Immunogen sequence location: 71-120
Reactive Species
A15101 is reactive to MRPL52 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
13.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15101 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HUVEC cells, using MRPL52 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRPL52 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-MRPL52 Antibody (A15101)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-MRPL52 Antibody
Question
I was wanting to use your anti-MRPL52 antibody for IHC for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
It shows on the product datasheet, A15101 anti-MRPL52 antibody has been tested for IF, IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-18
Question
Is a blocking peptide available for product anti-MRPL52 antibody (A15101)?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We do provide the blocking peptide for product anti-MRPL52 antibody (A15101). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-26
Question
I see that the anti-MRPL52 antibody A15101 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-05-15
Question
We are currently using anti-MRPL52 antibody A15101 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
The anti-MRPL52 antibody (A15101) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-04
Question
Does anti-MRPL52 antibody A15101 work for IHC with placenta?
Verified Customer
Verified customer
Asked: 2018-01-15
Answer
According to the expression profile of placenta, MRPL52 is highly expressed in placenta. So, it is likely that anti-MRPL52 antibody A15101 will work for IHC with placenta.
Boster Scientific Support
Answered: 2018-01-15
Question
Does anti-MRPL52 antibody A15101 work on canine IHC with left adrenal gland?
N. Gonzalez
Verified customer
Asked: 2017-09-04
Answer
Our lab technicians have not validated anti-MRPL52 antibody A15101 on canine. You can run a BLAST between canine and the immunogen sequence of anti-MRPL52 antibody A15101 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine left adrenal gland in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-04
