Anti-PRPF19 Antibody Picoband™

Product Name

Anti-PRPF19 Antibody Picoband™

View all PRP19 Antibodies

SKU/Catalog Number

A02434-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PRPF19 Antibody Picoband™ catalog # A02434-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PRPF19 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02434-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PRPF19 recombinant protein (Position: Q51-H359).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02434-3 is reactive to PRPF19 in Human

Applications

A02434-3 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

55.181kDa

Background of PRP19

Pre-mRNA-processing factor 19 is a protein that in humans is encoded by the PRPF19 gene. Enables identical protein binding activity and ubiquitin-ubiquitin ligase activity. Involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome. Acts upstream of or within protein polyubiquitination. Located in cytoplasm; nuclear speck; and site of double-strand break. Part of Prp19 complex and U2-type catalytic step 2 spliceosome. Colocalizes with DNA replication factor A complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PRPF19 using anti-PRPF19 antibody (A02434-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human MCF-7whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF19 antigen affinity purified polyclonal antibody (Catalog # A02434-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF19 at approximately 54 kDa. The expected band size for PRPF19 is at 55 kDa.

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Figure 2. IF analysis of PRPF19 using anti-PRPF19 antibody (A02434-3).
PRPF19 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRPF19 Antibody (A02434-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of JK cells using anti-PRPF19 antibody (A02434-3).
Overlay histogram showing JK cells stained with A02434-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF19 Antibody (A02434-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PRPF19 (Source: Uniprot.org, NCBI)

Alternative Names

hPSO4; NMP200SNEV; Nuclear matrix protein 200; nuclear matrix protein NMP200 related to splicing factor PRP19; pre-mRNA-processing factor 19; PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae); PRP19PRP19/PSO4 homolog (S. cerevisiae); PSO4PRP19/PSO4 homolog; Senescence evasion factor; UBOX4 PRPF19 NMP200, PRP19, PSO4, SNEV, UBOX4, hPSO4 pre-mRNA processing factor 19 pre-mRNA-processing factor 19|PRP19/PSO4 homolog|PRP19/PSO4 pre-mRNA processing factor 19 homolog|RING-type E3 ubiquitin transferase PRP19|nuclear matrix protein 200|nuclear matrix protein NMP200 related to splicing factor PRP19|psoralen 4|senescence evasion factor

For more info on PRPF19, check out the PRPF19 Infographic

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In this infographic, you will see the following information for PRPF19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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