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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human uPA / PLAU / URK ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human uPA / PLAU / URK ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLAU in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human uPA / PLAU / URK ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0535)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: M1-L431
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0535 is reactive to PLAU in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0535 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of u-Plasminogen Activator/Urokinase
Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).
|EK0535-CAP||Anti-Human PLAU Pre-coated 96-well strip microplate||1|
|EK0535-ST||Human PLAU Standard||2 vials, 10 ng/tube|
|EK0535-DA||Human PLAU Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human uPA/PLAU PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect u-Plasminogen Activator/Urokinase, Dilution ratio of 1:1, concentration in serum is 1000pg/ml 2000pg/ml.
Some articles we found to cite concentrations of u-Plasminogen Activator/Urokinase in samples: 20369484 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human uPA / PLAU / URK ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of u-Plasminogen Activator/Urokinase in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For PLAU (Source: Uniprot.Org, NCBI)
Urokinase-type plasminogen activator
peptidase S1 family
ATF; EC 3.4.21; EC 18.104.22.168; plasminogen activator, urokinase; PLAU; uPA; u-PA; uPlasminogen Activator; u-Plasminogen Activator; urinary; Urokinase; urokinase-type plasminogen activator PLAU ATF, BDPLT5, QPD, UPA, URK, u-PA plasminogen activator, urokinase urokinase-type plasminogen activator|U-plasminogen activator|plasminogen activator, urinary*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PLAU, check out the PLAU Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLAU: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0535 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα
Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13
miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?
Jiang Jt, Zhang Lf, Zhou B, Zhang Sq, Li Sm, Zhang W, Zhang J, Qiao Z, Kong Rr, Ma Yf, Chen S. Asian Pac J Cancer Prev. 2012;13(7):3379-83. Relationships Of Upa And Vegf Expression In Esophageal Cancer And Microvascular Density With Tumorous Invas...
Yang Y, Shi H, Li X, Yi Y. Oncol Rep. 2011 Jan;25(1):259-65. Effects Of Shrna Targeting Maspin On Invasion Of Gastric Carcinoma Sgc7901 Cell Line.
Wang R, Li Zq, Han X, Li Bl, Mi Xy, Sun Lm, Song M, Han Yc, Zhao Y, Wang Eh. Apmis. 2010 Dec;118(12):909-17. Doi: 10.1111/J.1600-0463.2010.02687.X. Epub 2010 Nov 2. Integrin ??3 And Its Ligand Regulate The Expression Of Upa Through P38 Mapk In Bre...
Bronckaers A, Hilkens P, Fanton Y, Struys T, Gervois P, Politis C, Martens W, Lambrichts I. Plos One. 2013 Aug 7;8(8):E71104. Doi: 10.1371/Journal.Pone.0071104. Ecollection 2013. Angiogenic Properties Of Human Dental Pulp Stem Cells.
Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13.
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