Human uPA / PLAU / URK ELISA Kit PicoKine™

Human uPA / PLAU / URK ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLAU in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. Cited in 6 publication(s).

Product Info Summary

SKU: EK0535
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human uPA / PLAU / URK ELISA Kit PicoKine™

See all u-Plasminogen Activator/Urokinase products

SKU/Catalog Number

EK0535

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human uPA / PLAU / URK ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLAU in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human uPA / PLAU / URK ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0535)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-L431

Sensitivity

<5 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0535 is reactive to PLAU in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0535 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0535-CAP Anti-Human PLAU Pre-coated 96-well strip microplate 1
EK0535-ST Human PLAU Standard 2 vials, 10 ng/tube
EK0535-DA Human PLAU Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0100.0890.1390.2500.6931.1141.7812.050

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect u-Plasminogen Activator/Urokinase, Dilution ratio of 1:1, concentration in serum is 1000pg/ml 2000pg/ml.

Some articles we found to cite concentrations of u-Plasminogen Activator/Urokinase in samples: 20369484 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human uPA / PLAU / URK ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)121158313732115361493
Standard deviation8.7739.0674.1412.8744.48103.01
CV (%)4.5%6.7%5.4%6.1%8.3%6.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of u-Plasminogen Activator/Urokinase in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11951831781911866.643.5%
Sample 258357154257856815.882.7%
Sample 31373141513221294135146.553.4%
*number of samples for each test n=16.

Gene/Protein Information For PLAU (Source: Uniprot.Org, NCBI)

Uniprot ID

P00749

Gene ID

5328

Gene Name

PLAU

Full Name

Urokinase-type plasminogen activator

Weight

48.507kDa

Superfamily

peptidase S1 family

Alternative Names

ATF; EC 3.4.21; EC 3.4.21.73; plasminogen activator, urokinase; PLAU; uPA; u-PA; uPlasminogen Activator; u-Plasminogen Activator; urinary; Urokinase; urokinase-type plasminogen activator PLAU ATF, BDPLT5, QPD, UPA, URK, u-PA plasminogen activator, urokinase urokinase-type plasminogen activator|U-plasminogen activator|plasminogen activator, urinary

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PLAU, check out the PLAU Infographic

PLAU infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PLAU: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0535 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?

Jiang Jt, Zhang Lf, Zhou B, Zhang Sq, Li Sm, Zhang W, Zhang J, Qiao Z, Kong Rr, Ma Yf, Chen S. Asian Pac J Cancer Prev. 2012;13(7):3379-83. Relationships Of Upa And Vegf Expression In Esophageal Cancer And Microvascular Density With Tumorous Invas...

Yang Y, Shi H, Li X, Yi Y. Oncol Rep. 2011 Jan;25(1):259-65. Effects Of Shrna Targeting Maspin On Invasion Of Gastric Carcinoma Sgc7901 Cell Line.

Wang R, Li Zq, Han X, Li Bl, Mi Xy, Sun Lm, Song M, Han Yc, Zhao Y, Wang Eh. Apmis. 2010 Dec;118(12):909-17. Doi: 10.1111/J.1600-0463.2010.02687.X. Epub 2010 Nov 2. Integrin ??3 And Its Ligand Regulate The Expression Of Upa Through P38 Mapk In Bre...

Bronckaers A, Hilkens P, Fanton Y, Struys T, Gervois P, Politis C, Martens W, Lambrichts I. Plos One. 2013 Aug 7;8(8):E71104. Doi: 10.1371/Journal.Pone.0071104. Ecollection 2013. Angiogenic Properties Of Human Dental Pulp Stem Cells.

Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13.

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Find answers in Q&As, reviews.

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11 Customer Q&As for Human uPA / PLAU / URK ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Z. Thompson

Verified customer

Asked: 2020-05-29

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-05-29

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Urokinase using the EK0535 Human Urokinase Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-04-02

Question

Have you tested this kit with normal (healthy) serum /plasma? Keyword: data, result

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

Yes, uPA level in healthy serum/plasma is about 423-5012 pg/ml.

Boster Scientific Support

Answered: 2019-05-14

Question

Q: if the enzyme conjugated Urokinase antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Urokinase antigen?

Verified Customer

Verified customer

Asked: 2019-04-22

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-04-22

Question

Q: is it okay to use citrate plasma as samples in Human Urokinase Picokine® ELISA Kit (Catalog # EK0535)?

S. Maharaj

Verified customer

Asked: 2019-03-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Urokinase causing disruption of its protein structure. Urokinase may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Urokinase ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-03-22

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-08-10

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-08-10

Question

Q: Can Urokinase ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-08-10

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-08-10

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Z. Li

Verified customer

Asked: 2018-06-30

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-06-30

Question

Q: how to proceed with the analysis of ELISA data? I have obtained Urokinase level in serum.

Verified Customer

Verified customer

Asked: 2018-06-21

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-06-21

Question

Q: how can I thaw whole blood sample for Urokinase ELISA after freezing?

Verified Customer

Verified customer

Asked: 2017-03-07

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Urokinase for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2017-03-07

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Urokinase in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

J. Babu

Verified customer

Asked: 2015-10-27

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Urokinase ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2015-10-27

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