Mouse Prolactin ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Mouse Prolactin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Prl in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ0594
Size: For 5 plates, 96 wells each
Reactive Species: Mouse
Application: ELISA

Product Name

Mouse Prolactin ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all Prolactin products

SKU/Catalog Number

EZ0594

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Mouse Prolactin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Prl in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Prolactin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0594)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L32-C228

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0594 is reactive to Prl in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, citrate).

Application Guarantee

EZ0594 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0594-CA Rabbit anti- mouse Prl polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0594-DA Biotinylated goat anti- mouse Prl polyclonal antibody (Detection Antibody) 500 µl, 3.6 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0594-ST Lyophilized recombinant mouse Prl standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.110.1850.2630.3980.6861.0471.6852.234

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)149029294543127226834726
Standard deviation67.05158.2177.299.22153246
CV (%)4.5%5.4%3.9%7.8%5.7%5.2%

Gene/Protein Information For Prl (Source: Uniprot.Org, NCBI)

Uniprot ID

P06879

Gene ID

19109

Gene Name

Prl

Full Name

Prolactin

Weight

25.496kDa

Superfamily

somatotropin/prolactin family

Alternative Names

PRL; Prolactin Prl|AV290867, Gha1, Pr1a1, Prl|prolactin|prolactin|growth hormone a1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Prl, check out the Prl Infographic

Prl infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Prl: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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