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Product Info Summary
| SKU: | M06794 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PSMA4 Mouse Monoclonal Antibody [Clone ID: OTI1H10]
SKU/Catalog Number
M06794
Size
100 µl
Description
Boster Bio PSMA4 mouse monoclonal antibody, clone OTI1H10 (formerly 1H10). Catalog# M06794. Tested in IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PSMA4 Mouse Monoclonal Antibody [Clone ID: OTI1H10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06794)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1H10
Isotype
IgG1
Immunogen
Full length human recombinant protein of human PSMA4 (NP_002780) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06794 is reactive to PSMA4 in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Observed Molecular Weight
29 kDa
Calculated molecular weight
29 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06794 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000, IHC 1:150
Positive Control
WB: human A549 whole cell, human MCF-7 whole cell, human 293T whole cell, rat liver tissue, rat brain tissue, mouse liver tissue, mouse brain tissue
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Figure 1. Western blot analysis of PSMA4 using anti-PSMA4 antibody (M06794).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PSMA4 antigen affinity purified monoclonal antibody (Catalog # M06794) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PSMA4 at approximately 29 kDa. The expected band size for PSMA4 is at 29 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PSMA4 (Source: Uniprot.org, NCBI)
Gene Name
PSMA4
Full Name
Proteasome subunit alpha type-4
Weight
29 kDa
Superfamily
peptidase T1A family
Alternative Names
HC9EC 3.4.25.1; HsT17706; Macropain subunit C9; MGC12467; MGC24813; Multicatalytic endopeptidase complex subunit C9; proteasome (prosome, macropain) subunit, alpha type, 4; Proteasome component C9; proteasome subunit alpha type-4; proteasome subunit HC9; Proteasome subunit L; PSC9MGC111191 PSMA4 HC9, HsT17706, PSC9 proteasome 20S subunit alpha 4 proteasome subunit alpha type-4|macropain subunit C9|multicatalytic endopeptidase complex subunit C9|proteasome (prosome, macropain) subunit, alpha type, 4|proteasome component C9|proteasome subunit HC9|proteasome subunit L|proteasome subunit alpha 4|proteasome subunit alpha3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSMA4, check out the PSMA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSMA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSMA4 Mouse Monoclonal Antibody [Clone ID: OTI1H10] (M06794)
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