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Product Info Summary
| SKU: | M06794 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PSMA4 Mouse Monoclonal Antibody [Clone ID: OTI1H10]
SKU/Catalog Number
M06794
Size
100 µl
Description
Boster Bio PSMA4 mouse monoclonal antibody, clone OTI1H10 (formerly 1H10). Catalog# M06794. Tested in IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PSMA4 Mouse Monoclonal Antibody [Clone ID: OTI1H10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06794)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1H10
Isotype
IgG1
Immunogen
Full length human recombinant protein of human PSMA4 (NP_002780) produced in HEK293T cell.
Reactive Species
M06794 is reactive to PSMA4 in Dog, Human, Monkey, Mouse, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
29.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06794 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500~2000
IHC: 1:150
Positive Control
WB: human A549 whole cell, human MCF-7 whole cell, human 293T whole cell, rat liver tissue, rat brain tissue, mouse liver tissue, mouse brain tissue
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Western blot analysis of PSMA4 using anti-PSMA4 antibody (M06794).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PSMA4 antigen affinity purified monoclonal antibody (Catalog # M06794) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PSMA4 at approximately 29 kDa. The expected band size for PSMA4 is at 29 kDa.
Specific Publications For Anti-PSMA4 Mouse Monoclonal Antibody [Clone ID: OTI1H10] (M06794)
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