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Product Info Summary
| SKU: | M07208 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PSMC3 Mouse Monoclonal Antibody [Clone ID: OTI9F3]
SKU/Catalog Number
M07208
Size
100 µl
Description
Boster Bio Anti-PSMC3 mouse monoclonal antibody, clone OTI9F3 (formerly 9F3). Catalog# M07208. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PSMC3 Mouse Monoclonal Antibody [Clone ID: OTI9F3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07208)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI9F3
Isotype
IgG1
Immunogen
Full length human recombinant protein of human PSMC3 (NP_002795) produced in HEK293T cell.
Reactive Species
M07208 is reactive to PSMC3 in Dog, Human, Mouse, Rat
Observed Molecular Weight
49 kDa
Calculated molecular weight
49.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07208 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:50
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells transfected with either pCMV6-ENTRY PSMC3 (Red) or empty vector control plasmid (Blue) were immunostained with anti-PSMC3 mouse monoclonal (M07208)
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Flow cytometric analysis of Jurkat cells
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Anti-PSMC3 mouse monoclonal antibody (M07208) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PSMC3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PSMC3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PSMC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.
Specific Publications For Anti-PSMC3 Mouse Monoclonal Antibody [Clone ID: OTI9F3] (M07208)
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Customer Q&As
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4 Customer Q&As for Anti-PSMC3 Mouse Monoclonal Antibody [Clone ID: OTI9F3]
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Proteasome 19S Rpt5/S6A Subunit antibody (Monoclonal, TBP1-19) M07208. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-25
Question
Will anti-Proteasome 19S Rpt5/S6A Subunit antibody (Monoclonal, TBP1-19) M07208 work on pig WB with liver?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Our lab technicians have not validated anti-Proteasome 19S Rpt5/S6A Subunit antibody (Monoclonal, TBP1-19) M07208 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Proteasome 19S Rpt5/S6A Subunit antibody (Monoclonal, TBP1-19) M07208 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
I would like to test anti-Proteasome 19S Rpt5/S6A Subunit antibody (Monoclonal, TBP1-19) M07208 on mouse liver for research purposes, then I may be interested in using anti-Proteasome 19S Rpt5/S6A Subunit antibody (Monoclonal, TBP1-19) M07208 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
The products we sell, including anti-Proteasome 19S Rpt5/S6A Subunit antibody (Monoclonal, TBP1-19) M07208, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-24
Question
We are currently using anti-Proteasome 19S Rpt5/S6A Subunit antibody (Monoclonal, TBP1-19) M07208 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
M. Thomas
Verified customer
Asked: 2018-12-31
Answer
The anti-Proteasome 19S Rpt5/S6A Subunit antibody (Monoclonal, TBP1-19) (M07208) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-31
