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Product Info Summary
| SKU: | MA1087 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PSTAIR Antibody (Monoclonal, PSTAIR)
SKU/Catalog Number
MA1087
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PSTAIR Antibody (Monoclonal, PSTAIR) catalog # MA1087. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PSTAIR Antibody (Monoclonal, PSTAIR) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1087)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
PSTAIR
Isotype
Mouse IgG1
Immunogen
Synthetic 16 amino acid oligopeptide containing the PSTAIR sequence conjugated to BSA.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1087 is reactive to CDK1 in Human, Mouse, Rat
Observed Molecular Weight
34 kDa
Calculated molecular weight
34.1 kDa
Background of CDK1
The cyclin-dependent protein kinases (CDKs) regulate major cell cycle transitions in eukaryotic cells. CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIRE motif found in prototypic CDC2 kinases. CDC2L1 is referred as PITSLRE B, based on the amino acid sequence of the region corresponding to the conserved CDC2 PSTAIRE box.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1087 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.5-1μg/ml, Human, mouse, rat
Positive Control
WB: human A549 whole cell, human 293T whole cell, human CACO-2 whole cell, human THP-1 whole cell, rat C6 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of PSTAIR using anti-PSTAIR antibody (MA1087).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PSTAIR antigen affinity purified monoclonal antibody (Catalog # MA1087) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PSTAIR at approximately 34 kDa. The expected band size for PSTAIR is at 34 kDa.
Specific Publications For Anti-PSTAIR Antibody (Monoclonal, PSTAIR) (MA1087)
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Customer Q&As
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15 Customer Q&As for Anti-PSTAIR Antibody (Monoclonal, PSTAIR)
Question
Will MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
As indicated on the product datasheet, MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-12
Question
Will anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 work on pig WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Our lab technicians have not validated anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
Would anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 work for WB with intestine?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
According to the expression profile of intestine, CDK1 is highly expressed in intestine. So, it is likely that anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 will work for WB with intestine.
Boster Scientific Support
Answered: 2019-12-20
Question
We want to test anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 on mouse intestine for research purposes, then I may be interested in using anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
The products we sell, including anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-01
Question
We are currently using anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The anti-CDK1 antibody (Monoclonal, PSTAIR) (MA1087) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-30
Question
We have observed staining in mouse cervix carcinoma. Do you have any suggestions? Is anti-CDK1 antibody (Monoclonal, PSTAIR) supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
According to literature cervix carcinoma does express CDK1. According to Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-23
Question
I was wanting to use your anti-CDK1 antibody (Monoclonal, PSTAIR) for WB for mouse intestine on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse intestine identification?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
You can see on the product datasheet, MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR) has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-09
Question
Is there a BSA free version of anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 available?
M. Williams
Verified customer
Asked: 2019-07-12
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-12
Question
We were well pleased with the WB result of your anti-CDK1 antibody (Monoclonal, PSTAIR). However we have seen positive staining in cervix carcinoma nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
From what I have seen in literature, cervix carcinoma does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-12-07
Question
I see that the anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-04-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for intestine using anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-18
Question
Is a blocking peptide available for product anti-CDK1 antibody (Monoclonal, PSTAIR) (MA1087)?
Z. Miller
Verified customer
Asked: 2016-06-14
Answer
We do provide the blocking peptide for product anti-CDK1 antibody (Monoclonal, PSTAIR) (MA1087). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-06-14
Question
My question regarding product MA1087, anti-CDK1 antibody (Monoclonal, PSTAIR). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Johnson
Verified customer
Asked: 2015-03-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-03-19
Question
Is this MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR) reactive to the isotypes of CDK1?
J. Banerjee
Verified customer
Asked: 2014-02-21
Answer
The immunogen of MA1087 anti-CDK1 antibody (Monoclonal, PSTAIR) is Synthetic 16 amino acid oligopeptide containing the CDK1 sequence conjugated to BSA. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-21
Question
Here is the WB image, lot number and protocol we used for intestine using anti-CDK1 antibody (Monoclonal, PSTAIR) MA1087. Please let me know if you require anything else.
M. Walker
Verified customer
Asked: 2013-10-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-15
