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Product Info Summary
| SKU: | A00006-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PTEN Antibody Picoband®
SKU/Catalog Number
A00006-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTEN Antibody Picoband® catalog # A00006-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTEN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00006-1)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PTEN recombinant protein (Position: T2-T202). Human PTEN shares 100% amino acid (aa) sequence identity with mouse PTEN.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00006-1 is reactive to PTEN in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
47.2 kDa
Background of PTEN
PTEN is also known as BZS, DEC, CWS1, GLM2, MHAM, TEP1, PTEN1. It is mapped to 10q23.3. This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. The PTEN structure reveals a phosphatase domain that is similar to protein phosphatases but also has an enlarged active site important for the accommodation of the phosphoinositide substrate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00006-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse liver tissue, human A549 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PTEN using anti-PTEN antibody (A00006-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse liver tissue lysate,
Lane 3: human A549 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN antigen affinity purified polyclonal antibody (Catalog # A00006-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTEN at approximately 54KD. The expected band size for PTEN is at 47KD.
Specific Publications For Anti-PTEN Antibody Picoband® (A00006-1)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PTEN Antibody Picoband®
Question
We have seen staining in mouse liver. Do you have any suggestions? Is anti-PTEN antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
From what I have seen in literature liver does express PTEN. From what I have seen in Uniprot.org, PTEN is expressed in intestine, epithelium, spleen, lung, liver, among other tissues. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-PTEN antibody A00006-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-14
Question
We were satisfied with the WB result of your anti-PTEN antibody. However we have been able to see positive staining in intestine cytoplasm using this antibody. Is that expected? Could you tell me where is PTEN supposed to be expressed?
O. Edwards
Verified customer
Asked: 2019-09-27
Answer
From what I have seen in literature, intestine does express PTEN. Generally PTEN expresses in cytoplasm. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-27
Question
I was wanting to use your anti-PTEN antibody for WB for mouse lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
It shows on the product datasheet, A00006-1 anti-PTEN antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-19
Question
Would A00006-1 anti-PTEN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
You can see on the product datasheet, A00006-1 anti-PTEN antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-08
Question
Does anti-PTEN antibody A00006-1 work for WB with lung?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
According to the expression profile of lung, PTEN is highly expressed in lung. So, it is likely that anti-PTEN antibody A00006-1 will work for WB with lung.
Boster Scientific Support
Answered: 2019-07-05
Question
I would like to test anti-PTEN antibody A00006-1 on mouse lung for research purposes, then I may be interested in using anti-PTEN antibody A00006-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
The products we sell, including anti-PTEN antibody A00006-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-15
Question
I see that the anti-PTEN antibody A00006-1 works with WB, what is the protocol used to produce the result images on the product page?
E. Jha
Verified customer
Asked: 2018-09-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-09-14
Question
I have a question about product A00006-1, anti-PTEN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00006-1 anti-PTEN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-27
Question
I have attached the WB image, lot number and protocol we used for lung using anti-PTEN antibody A00006-1. Please let me know if you require anything else.
M. Jackson
Verified customer
Asked: 2017-02-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-22
Question
Is a blocking peptide available for product anti-PTEN antibody (A00006-1)?
J. Wu
Verified customer
Asked: 2017-02-06
Answer
We do provide the blocking peptide for product anti-PTEN antibody (A00006-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-02-06
Question
you antibody using your anti-PTEN antibody for negative regulation of synaptic vesicle clustering studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
R. Jones
Verified customer
Asked: 2016-11-02
Answer
Thanks for your inquiry. This A00006-1 anti-PTEN antibody is validated on rat brain tissue, tissue lysate, mouse liver tissue, human a549, a549 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-11-02
Question
We are currently using anti-PTEN antibody A00006-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
F. Kulkarni
Verified customer
Asked: 2015-09-08
Answer
The anti-PTEN antibody (A00006-1) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-08
Question
Do you have a BSA free version of anti-PTEN antibody A00006-1 available?
G. Baker
Verified customer
Asked: 2014-08-14
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PTEN antibody A00006-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-08-14
Question
Is this A00006-1 anti-PTEN antibody reactive to the isotypes of PTEN?
G. Bhatt
Verified customer
Asked: 2013-11-19
Answer
The immunogen of A00006-1 anti-PTEN antibody is E. coli-derived human PTEN recombinant protein (Position: T2-T202). Human PTEN shares 100% amino acid (aa) sequence identity with mouse PTEN. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-11-19
