Anti-PTEN Antibody Picoband™

PTEN antibody

Boster Bio Anti-PTEN Antibody Picoband™ catalog # A00006-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: A00006-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-PTEN Antibody Picoband™

View all PTEN Antibodies

SKU/Catalog Number

A00006-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PTEN Antibody Picoband™ catalog # A00006-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PTEN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00006-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human PTEN recombinant protein (Position: T2-T202). Human PTEN shares 100% amino acid (aa) sequence identity with mouse PTEN.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00006-1 is reactive to PTEN in Human, Mouse, Rat

Applications

A00006-1 is guaranteed for WB Boster Guarantee

Background of PTEN

PTEN is also known as BZS, DEC, CWS1, GLM2, MHAM, TEP1, PTEN1. It is mapped to 10q23.3. This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. The PTEN structure reveals a phosphatase domain that is similar to protein phosphatases but also has an enlarged active site important for the accommodation of the phosphoinositide substrate.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For PTEN (Source: Uniprot.org, NCBI)

Uniprot ID

P60484

Gene ID

5728

Gene Name

PTEN

Full Name

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

Weight

47.166kDa

Superfamily

PTEN phosphatase protein family

Alternative Names

BZS; EC 3.1.3.16,10q23del; EC 3.1.3.48; EC 3.1.3.67; GLM2; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; MMAC1; MMAC1MGC11227; Mutated in multiple advanced cancers 1; phosphatase and tensin homologDEC; phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN; PTEN; PTEN1; TEP1MHAM PTEN 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC11, PTENbeta, TEP1, PTEN phosphatase and tensin homolog phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|MMAC1 phosphatase and tensin homolog deleted on chromosome 10|PTENepsilon|mitochondrial PTENalpha|mitochondrial phosphatase and tensin protein alpha|mutated in multiple advanced cancers 1|phosphatase and tensin-like protein|phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|protein tyrosine phosphatase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PTEN, check out the PTEN Infographic

PTEN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTEN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

A00006-1 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

miR-223 reverses the resistance of EGFR-TKIs through IGF1R/PI3K/Akt signaling pathway

Sun M,Hu L, S,Huang T,Zhang M,Yang M,Zhen W,Yang D,Lu W,Guan M,Peng S. Circulating MicroRNA-19b Identified From Osteoporotic Vertebral Compression Fracture Patients Increases Bone Formation. J Bone Miner Res.2020 Feb;35(2):306-316. doi:10.1002/jbmr.3892.
Species: Human,Mouse
A00006-1 usage in article: APP:WB, SAMPLE:HMSCS CELL AND MC3T3-E1 CELL, DILUTION:1:500

Long-term effects of repeated superovulation on the uterus and mammary gland in rhesus monkeys

Study on the expression and mechanism of inflammatory factors in the brain of rats with cerebral vasospasm

miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit

miR-21 increases c-kit cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling

Expression and significance of PTEN, hypoxia-inducible factor-1 alpha in colorectal adenoma and adenocarcinoma

Prognostic role of PPAR-%u03B3 and PTEN in the renal cell carcinoma

Pin1 Promotes Regulated Necrosis Induced by Glutamate in Rat Retinal Neurons via CAST/Calpain2 Pathway

708: Combined Analysis of EGFR and PTEN Status in Patients With KRAS Wild-Type Metastatic Colorectal Cancer

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Have a question?

Find answers in Q&As, reviews.

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15 Customer Q&As for Anti-PTEN Antibody Picoband™

Question

We have seen staining in mouse liver. Do you have any suggestions? Is anti-PTEN antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

From what I have seen in literature liver does express PTEN. From what I have seen in Uniprot.org, PTEN is expressed in intestine, epithelium, spleen, lung, liver, among other tissues. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-01-07

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-PTEN antibody A00006-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-14

Question

We were satisfied with the WB result of your anti-PTEN antibody. However we have been able to see positive staining in intestine cytoplasm using this antibody. Is that expected? Could you tell me where is PTEN supposed to be expressed?

O. Edwards

Verified customer

Asked: 2019-09-27

Answer

From what I have seen in literature, intestine does express PTEN. Generally PTEN expresses in cytoplasm. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-09-27

Question

I was wanting to use your anti-PTEN antibody for WB for mouse lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung identification?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

It shows on the product datasheet, A00006-1 anti-PTEN antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-19

Question

Would A00006-1 anti-PTEN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

You can see on the product datasheet, A00006-1 anti-PTEN antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-08

Question

Does anti-PTEN antibody A00006-1 work for WB with lung?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

According to the expression profile of lung, PTEN is highly expressed in lung. So, it is likely that anti-PTEN antibody A00006-1 will work for WB with lung.

Boster Scientific Support

Answered: 2019-07-05

Question

I would like to test anti-PTEN antibody A00006-1 on mouse lung for research purposes, then I may be interested in using anti-PTEN antibody A00006-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-11-15

Answer

The products we sell, including anti-PTEN antibody A00006-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-11-15

Question

I see that the anti-PTEN antibody A00006-1 works with WB, what is the protocol used to produce the result images on the product page?

E. Jha

Verified customer

Asked: 2018-09-14

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-09-14

Question

I have a question about product A00006-1, anti-PTEN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-06-27

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00006-1 anti-PTEN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-27

Question

I have attached the WB image, lot number and protocol we used for lung using anti-PTEN antibody A00006-1. Please let me know if you require anything else.

M. Jackson

Verified customer

Asked: 2017-02-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-02-22

Question

Is a blocking peptide available for product anti-PTEN antibody (A00006-1)?

J. Wu

Verified customer

Asked: 2017-02-06

Answer

We do provide the blocking peptide for product anti-PTEN antibody (A00006-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-02-06

Question

you antibody using your anti-PTEN antibody for negative regulation of synaptic vesicle clustering studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

R. Jones

Verified customer

Asked: 2016-11-02

Answer

Thanks for your inquiry. This A00006-1 anti-PTEN antibody is validated on rat brain tissue, tissue lysate, mouse liver tissue, human a549, a549 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-11-02

Question

We are currently using anti-PTEN antibody A00006-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?

F. Kulkarni

Verified customer

Asked: 2015-09-08

Answer

The anti-PTEN antibody (A00006-1) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-09-08

Question

Do you have a BSA free version of anti-PTEN antibody A00006-1 available?

G. Baker

Verified customer

Asked: 2014-08-14

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PTEN antibody A00006-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-08-14

Question

Is this A00006-1 anti-PTEN antibody reactive to the isotypes of PTEN?

G. Bhatt

Verified customer

Asked: 2013-11-19

Answer

The immunogen of A00006-1 anti-PTEN antibody is E. coli-derived human PTEN recombinant protein (Position: T2-T202). Human PTEN shares 100% amino acid (aa) sequence identity with mouse PTEN. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-11-19

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Total: $315

SKU:A00006-1

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A00006-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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