Anti-Rab 25 Monoclonal Antibody

Boster Bio Anti-Rab 25 Monoclonal Antibody catalog # A05115. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A05115
Size: 100μl
Reactive Species: Human, Mouse
Host: Mouse
Application: Flow Cytometry, IF, IHC, WB

Product Name

Anti-Rab 25 Monoclonal Antibody

See all RAB25 products

SKU/Catalog Number

A05115

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Rab 25 Monoclonal Antibody catalog # A05115. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Rab 25 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05115)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clone Number

3F12

Immunogen

Purified recombinant fragment of Rab 25 expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05115 is reactive to RAB25 in Human, Mouse

Applications

A05115 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC, 1:200-1:1000
IF, 1:200-1:1000
FC, 1:200-1:400

Validation Images & Assay Conditions

Gene/Protein Information For RAB25 (Source: Uniprot.Org, NCBI)

Uniprot ID

P57735

Gene ID

57111

Gene Name

RAB25

Full Name

Ras-related protein Rab-25

Weight

23.496kDa

Superfamily

small GTPase superfamily

Alternative Names

CATX8; CATX-8RAB11C; RAB11C; Rab25; RAB25, member RAS oncogene family; ras-related protein Rab-25 RAB25 CATX-8, RAB11C RAB25, member RAS oncogene family ras-related protein Rab-25

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RAB25, check out the RAB25 Infographic

RAB25 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RAB25: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A05115

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4 Customer Q&As for Anti-Rab 25 Monoclonal Antibody

Question

Can you help my question with product A05115, anti-Rab 25 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05115 anti-Rab 25 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-03

Question

Is this A05115 anti-Rab 25 Monoclonal antibody reactive to the isotypes of RAB25?

J. Banerjee

Verified customer

Asked: 2020-02-04

Answer

The immunogen of A05115 anti-Rab 25 Monoclonal antibody is Purified recombinant fragment of Rab 25 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-04

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus mucosa using anti-Rab 25 Monoclonal antibody A05115. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-23

Question

We are currently using anti-Rab 25 Monoclonal antibody A05115 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2017-08-01

Answer

The anti-Rab 25 Monoclonal antibody (A05115) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-08-01

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A05115
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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