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Product Info Summary
| SKU: | A01916-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Protein C inhibitor/SERPINA5 Antibody Picoband®
SKU/Catalog Number
A01916-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Protein C inhibitor/SERPINA5 Antibody Picoband® catalog # A01916-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein C inhibitor/SERPINA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01916-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SERPINA5 recombinant protein (Position: S86-P406). Human SERPINA5 shares 66% amino acid (aa) sequence identity with mouse SERPINA5.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A01916-1 is reactive to SERPINA5 in Human, Mouse, Rat
Observed Molecular Weight
46 kDa
Calculated molecular weight
45.7 kDa
Background of SERPINA5
Protein C inhibitor (PCI), also known as SERPINA5, is serine protease inhibitor of serpin type that is found in most tissues and fluids, including blood plasma, seminal plasma and urine of human. It is a 52kD glycoprotein and belongs to serine protease inhibitor (Serpin) super family of protein. This family member is a glycoprotein that can inhibit several serine proteases, including protein C and various plasminogen activators and kallikreins, and it thus plays diverse roles in hemostasis and thrombosis in multiple organs. This gene is mapped on the q arm of chromosome 14.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01916-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat liver tissue, human SKOV3 whole cell
IHC: mouse kidney tissue, rat kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of SERPINA5 using anti-SERPINA5 antibody (A01916-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: human SKOV3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA5 antigen affinity purified polyclonal antibody (Catalog # A01916-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA5 at approximately 46 kDa. The expected band size for SERPINA5 is at 46KD.
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IHC analysis of SERPINA5 using anti-SERPINA5 antibody (A01916-1).
SERPINA5 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SERPINA5 Antibody (A01916-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of SERPINA5 using anti-SERPINA5 antibody (A01916-1).
SERPINA5 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SERPINA5 Antibody (A01916-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Protein C inhibitor/SERPINA5 Antibody Picoband® (A01916-1)
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3 Customer Q&As for Anti-Protein C inhibitor/SERPINA5 Antibody Picoband®
Question
My question regarding product A01916-1, anti-Protein C inhibitor/SERPINA5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Moore
Verified customer
Asked: 2019-10-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01916-1 anti-Protein C inhibitor/SERPINA5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-29
Question
We are currently using anti-Protein C inhibitor/SERPINA5 antibody A01916-1 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
K. Jha
Verified customer
Asked: 2018-11-02
Answer
The anti-Protein C inhibitor/SERPINA5 antibody (A01916-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-02
Question
Would anti-Protein C inhibitor/SERPINA5 antibody A01916-1 work on canine WB with testis?
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
Our lab technicians have not validated anti-Protein C inhibitor/SERPINA5 antibody A01916-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Protein C inhibitor/SERPINA5 antibody A01916-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-16
