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Product Info Summary
| SKU: | A07244-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Rab 3 GAP p150 RAB3GAP2 Antibody
SKU/Catalog Number
A07244-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rab 3 GAP p150 RAB3GAP2 Antibody catalog # A07244-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab 3 GAP p150 RAB3GAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07244-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RAB3GAP2. AA range:417-466
Reactive Species
A07244-1 is reactive to RAB3GAP2 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
156.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07244-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Rab 3 GAP p150 Polyclonal Antibody
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Western blot analysis of lysates from COS cells, using RAB3GAP2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RAB3GAP2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Rab 3 GAP p150 RAB3GAP2 Antibody (A07244-1)
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Customer Q&As
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6 Customer Q&As for Anti-Rab 3 GAP p150 RAB3GAP2 Antibody
Question
Does anti-Rab 3 GAP p150 antibody A07244-1 work on feline WB with brain?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Our lab technicians have not validated anti-Rab 3 GAP p150 antibody A07244-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Rab 3 GAP p150 antibody A07244-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-21
Question
Can you help my question with product A07244-1, anti-Rab 3 GAP p150 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07244-1 anti-Rab 3 GAP p150 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-05
Question
I was wanting to use your anti-Rab 3 GAP p150 antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
As indicated on the product datasheet, A07244-1 anti-Rab 3 GAP p150 antibody has been validated for WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-09
Question
Do you have a BSA free version of anti-Rab 3 GAP p150 antibody A07244-1 available?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rab 3 GAP p150 antibody A07244-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-04
Question
I see that the anti-Rab 3 GAP p150 antibody A07244-1 works with WB, what is the protocol used to produce the result images on the product page?
B. Patel
Verified customer
Asked: 2018-07-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-07-16
Question
Is this A07244-1 anti-Rab 3 GAP p150 antibody reactive to the isotypes of RAB3GAP2?
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
The immunogen of A07244-1 anti-Rab 3 GAP p150 antibody is Synthesized peptide derived from the Internal region of human Rab 3 GAP p150. at AA rangle: 390-470. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-03
