Product Info Summary
SKU: | PB9322 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Rab3A Antibody Picoband®
SKU/Catalog Number
PB9322
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rab3A Antibody Picoband® catalog # PB9322. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab3A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9322)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab3A, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9322 is reactive to RAB3A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
24984 MW
Background of RAB3A
Ras-related protein Rab-3A is a protein that in humans is encoded by the RAB3A gene. This gene is mapped to 19p13.11. Synapsin I stimulated the RAB3A cycle by increasing GTP binding, GTPase activity, and RAB3A recruitment to the synaptic vesicle membrane. Conversely, RAB3A inhibited synapsin I binding to actin and synapsin I-induced synaptic vesicle clustering. It has been found that RAB3A was necessary for both forms of synaptic plasticity, and it is also involved in calcium exocytosis in neurons. In addition to that, RAB3A has been found to regulate a late step in synaptic vesicle fusion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9322 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Rab3A using anti-Rab3A antibody (PB9322).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab3A antigen affinity purified polyclonal antibody (Catalog # PB9322) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab3A at approximately 25 kDa. The expected band size for Rab3A is at 25 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RAB3A (Source: Uniprot.org, NCBI)
Gene Name
RAB3A
Full Name
Ras-related protein Rab-3A
Weight
24984 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-3A;RAB3A; RAB3A RAB3A, member RAS oncogene family ras-related protein Rab-3A|RAS-associated protein RAB3A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB3A, check out the RAB3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab3A Antibody Picoband® (PB9322)
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Customer Q&As
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1 Customer Q&As for Anti-Rab3A Antibody Picoband®
Question
We are currently using anti-Rab3A antibody PB9322 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The anti-Rab3A antibody (PB9322) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-13