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Product Info Summary
| SKU: | A09291 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Rabenosyn-5 RBSN Antibody
SKU/Catalog Number
A09291
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rabenosyn-5 RBSN Antibody catalog # A09291. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rabenosyn-5 RBSN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09291)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ZFYVE20. AA range:145-194
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09291 is reactive to RBSN in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
88.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09291 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of various cells using Rabenosyn-5 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from HUVEC and Jurkat cells, using ZFYVE20 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from COLO205 cells using ZFYVE20 antibody.
Specific Publications For Anti-Rabenosyn-5 RBSN Antibody (A09291)
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Customer Q&As
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4 Customer Q&As for Anti-Rabenosyn-5 RBSN Antibody
Question
Our lab want to know about to test anti-Rabenosyn-5 antibody A09291 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-Rabenosyn-5 antibody A09291 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
The products we sell, including anti-Rabenosyn-5 antibody A09291, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-23
Question
We are currently using anti-Rabenosyn-5 antibody A09291 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The anti-Rabenosyn-5 antibody (A09291) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-09
Question
Will anti-Rabenosyn-5 antibody A09291 work on feline WB with liver?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Our lab technicians have not tested anti-Rabenosyn-5 antibody A09291 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Rabenosyn-5 antibody A09291 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-31
Question
Do you have a BSA free version of anti-Rabenosyn-5 antibody A09291 available?
S. Wu
Verified customer
Asked: 2018-02-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rabenosyn-5 antibody A09291 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-07
