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Product Info Summary
| SKU: | M09291 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ZFYVE20 Antibody (N-Term)
SKU/Catalog Number
M09291
Size
80 µl
Description
Boster Bio Anti-ZFYVE20 Antibody (N-Term) (Catalog # M09291). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ZFYVE20 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09291)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ZFYVE20 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 41-65 amino acids from the human region of human ZFYVE20.
Reactive Species
M09291 is reactive to RBSN in Human
Calculated molecular weight
88.9 kDa
Background of RBSN
Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3) (PubMed:11062261, PubMed:11788822, PubMed:15020713). Plays a role in the recycling of transferrin receptor to the plasma membrane (PubMed:22308388).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09291 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-ZFYVE20 Antibody (N-Term) at 1:8000 dilution
Lane 1: Hela whole cell lysates
Lane 2: K562 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 89 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-ZFYVE20 Antibody (N-Term)at 1:2000 dilution + A431 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 89 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-ZFYVE20 Antibody (N-Term) (M09291)
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