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Product Info Summary
| SKU: | A10247 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Rabphilin-3A RPH3A Antibody
SKU/Catalog Number
A10247
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rabphilin-3A RPH3A Antibody catalog # A10247. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rabphilin-3A RPH3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10247)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Rabphilin 3A. AA range:203-252
Reactive Species
A10247 is reactive to RPH3A in Human, Mouse, Rat
Calculated molecular weight
76.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10247 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Rabphilin-3A Polyclonal Antibody
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Western blot analysis of lysates from HeLa cells, treated with TNF-a 20ng/ml 2', using Rabphilin 3A Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Rabphilin 3A Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using Rabphilin 3A Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Rabphilin-3A RPH3A Antibody (A10247)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Rabphilin-3A RPH3A Antibody
Question
Will anti-Rabphilin-3A antibody A10247 work for WB with frontal cortex?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
According to the expression profile of frontal cortex, RPH3A is highly expressed in frontal cortex. So, it is likely that anti-Rabphilin-3A antibody A10247 will work for WB with frontal cortex.
Boster Scientific Support
Answered: 2019-11-29
Question
See attached the WB image, lot number and protocol we used for frontal cortex using anti-Rabphilin-3A antibody A10247. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-13
Question
Is this A10247 anti-Rabphilin-3A antibody reactive to the isotypes of RPH3A?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The immunogen of A10247 anti-Rabphilin-3A antibody is Synthesized peptide derived from human Rabphilin-3A around the non-phosphorylation site of S237. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-26
Question
Does anti-Rabphilin-3A antibody A10247 work on dog WB with hippocampus?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
Our lab technicians have not validated anti-Rabphilin-3A antibody A10247 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Rabphilin-3A antibody A10247 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog hippocampus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for frontal cortex using anti-Rabphilin-3A antibody A10247. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-06
Question
I was wanting to use your anti-Rabphilin-3A antibody for WB for mouse frontal cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse frontal cortex identification?
B. Li
Verified customer
Asked: 2014-08-05
Answer
As indicated on the product datasheet, A10247 anti-Rabphilin-3A antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse frontal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-05
