Anti-Rac1/CDC42 Antibody

Rac1 antibody

Boster Bio Anti-Rac1/CDC42 Antibody (Catalog# A03252). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A03252
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, WB, IF (paraffin)

Product Name

Anti-Rac1/CDC42 Antibody

View all Rac1 Antibodies

SKU/Catalog Number

A03252

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Rac1/CDC42 Antibody (Catalog# A03252). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Rac1/CDC42 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03252)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human Rac1/CDC42. Immunogen sequence location: 38-87

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03252 is reactive to RAC1 in Human, Mouse, Rat

Applications

A03252 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

21.45kDa

Background of Rac1

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For RAC1 (Source: Uniprot.org, NCBI)

Gene Name

RAC1

Full Name

Ras-related C3 botulinum toxin substrate 1

Weight

21.45kDa

Superfamily

small GTPase superfamily

Alternative Names

Cell migration-inducing gene 5 protein; MGC111543; p21-Rac1; p21-Rac1migration-inducing gene 5; Rac1; Ras-like protein TC25; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP bindingprotein Rac1); ras-related C3 botulinum toxin substrate 1; rho family, small GTP binding protein Rac1; TC25; TC-25 RAC1 MIG5, MRD48, Rac-1, TC-25, p21-Rac1 Rac family small GTPase 1 ras-related C3 botulinum toxin substrate 1|cell migration-inducing gene 5 protein|ras-like protein TC25|ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RAC1, check out the RAC1 Infographic

RAC1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A03252

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Rac1/CDC42 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Rac1/CDC42 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-Rac1/CDC42 Antibody

Question

Will A03252 anti-Rac1/CDC42 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

It shows on the product datasheet, A03252 anti-Rac1/CDC42 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-01

Question

Is a blocking peptide available for product anti-Rac1/CDC42 antibody (A03252)?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

We do provide the blocking peptide for product anti-Rac1/CDC42 antibody (A03252). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-14

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-Rac1/CDC42 antibody A03252. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-22

Question

you antibody to test anti-Rac1/CDC42 antibody A03252 on human melanoma for research purposes, then I may be interested in using anti-Rac1/CDC42 antibody A03252 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

The products we sell, including anti-Rac1/CDC42 antibody A03252, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-13

Question

Is there a BSA free version of anti-Rac1/CDC42 antibody A03252 available?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rac1/CDC42 antibody A03252 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-17

Question

We ordered your anti-Rac1/CDC42 antibody for IHC on melanoma in a previous project. I am using mouse, and We intend to use the antibody for WB next. We are interested in examining melanoma as well as colon skin in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

I looked at the website and datasheets of our anti-Rac1/CDC42 antibody and it seems that A03252 has been validated on mouse in both IHC and WB. Thus A03252 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-23

Question

I would like using your anti-Rac1/CDC42 antibody for regulation of stress fiber assembly studies. Has this antibody been tested with western blotting on breast carcinoma tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

Thanks for your inquiry. This A03252 anti-Rac1/CDC42 antibody is validated on breast carcinoma tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-16

Question

My team were happy with the WB result of your anti-Rac1/CDC42 antibody. However we have been able to see positive staining in melanoma cell membrane using this antibody. Is that expected? Could you tell me where is RAC1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

Based on literature, melanoma does express RAC1. Generally RAC1 expresses in cell membrane. Regarding which tissues have RAC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Colon, and Skin, Pubmed ID: 10597294
Melanoma, Pubmed ID: 17081065
Pancreas, and Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-05

Question

Is this A03252 anti-Rac1/CDC42 antibody reactive to the isotypes of RAC1?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

The immunogen of A03252 anti-Rac1/CDC42 antibody is A peptide derived from human Rac1/CDC42. Immunogen sequence location: 38-87. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-21

Question

Here is the WB image, lot number and protocol we used for melanoma using anti-Rac1/CDC42 antibody A03252. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-04-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-19

Question

We have been able to see staining in human melanoma. Any tips? Is anti-Rac1/CDC42 antibody supposed to stain melanoma positively?

Verified Customer

Verified customer

Asked: 2019-01-17

Answer

From what I have seen in literature melanoma does express RAC1. From what I have seen in Uniprot.org, RAC1 is expressed in esophagus squamous epithelium, colon skin, pancreas skin, melanoma, cervix carcinoma, among other tissues. Regarding which tissues have RAC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Colon, and Skin, Pubmed ID: 10597294
Melanoma, Pubmed ID: 17081065
Pancreas, and Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-01-17

Question

We are currently using anti-Rac1/CDC42 antibody A03252 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

R. Krishna

Verified customer

Asked: 2018-08-07

Answer

The anti-Rac1/CDC42 antibody (A03252) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-07

Question

My question regarding product A03252, anti-Rac1/CDC42 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Krishna

Verified customer

Asked: 2016-12-06

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03252 anti-Rac1/CDC42 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-12-06

Question

Will anti-Rac1/CDC42 antibody A03252 work for IHC with melanoma?

D. Li

Verified customer

Asked: 2016-08-31

Answer

According to the expression profile of melanoma, RAC1 is highly expressed in melanoma. So, it is likely that anti-Rac1/CDC42 antibody A03252 will work for IHC with melanoma.

Boster Scientific Support

Answered: 2016-08-31

Question

I see that the anti-Rac1/CDC42 antibody A03252 works with IHC, what is the protocol used to produce the result images on the product page?

O. Johnson

Verified customer

Asked: 2016-02-03

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-02-03

Question

I was wanting to use your anti-Rac1/CDC42 antibody for IHC for human melanoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human melanoma identification?

A. Anderson

Verified customer

Asked: 2013-05-27

Answer

As indicated on the product datasheet, A03252 anti-Rac1/CDC42 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human melanoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-05-27

Order DetailsPrice
A03252

100 μL

$335

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A03252
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.