Product Info Summary
SKU: | M00088 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-Rad51 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00088
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rad51 Rabbit Monoclonal Antibody catalog # M00088. Tested in WB, Flow Cytometry, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rad51 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00088)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CAE-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rad51
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00088 is reactive to RAD51 in Human, Mouse, Rat
Applications
M00088 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
36.966kDa
Background of RAD51
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Rad51 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RAD51 (Source: Uniprot.org, NCBI)
Gene Name
RAD51
Full Name
DNA repair protein RAD51 homolog 1
Weight
36.966kDa
Superfamily
RecA family
Alternative Names
BRCC5; HRAD51; HsRad51; HsT16930; RAD51 (S. cerevisiae) homolog (E coli RecA homolog); RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RAD51 homolog A; Rad51; RAD51ABRCA1/BRCA2-containing complex, subunit 5; RAD51L3; RecA, E. coli, homolog of; RECADNA repair protein RAD51 homolog 1; RecA-like protein; recombination protein A RAD51 BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2A, RECA, RAD51 RAD51 recombinase DNA repair protein RAD51 homolog 1|BRCA1/BRCA2-containing complex, subunit 5|RAD51 homolog A|RecA, E. coli, homolog of|RecA-like protein|recombination protein A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAD51, check out the RAD51 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAD51: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rad51 Rabbit Monoclonal Antibody (M00088)
Hello CJ!
No publications found for M00088
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Rad51 Rabbit Monoclonal Antibody
Question
Would M00088 anti-Rad51 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
It shows on the product datasheet, M00088 anti-Rad51 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-16
Question
We ordered your anti-Rad51 Rabbit Monoclonal antibody for IP on embryo in a previous experiment. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining embryo as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
I have checked the website and datasheets of our anti-Rad51 Rabbit Monoclonal antibody and it appears that M00088 has been tested on mouse in both IP and WB. Thus M00088 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-14
Question
I see that the anti-Rad51 Rabbit Monoclonal antibody M00088 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-28
Question
Is a blocking peptide available for product anti-Rad51 Rabbit Monoclonal antibody (M00088)?
K. Banerjee
Verified customer
Asked: 2020-01-15
Answer
We do provide the blocking peptide for product anti-Rad51 Rabbit Monoclonal antibody (M00088). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-15
Question
Is there a BSA free version of anti-Rad51 Rabbit Monoclonal antibody M00088 available?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rad51 Rabbit Monoclonal antibody M00088 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-19
Question
Can you help my question with product M00088, anti-Rad51 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00088 anti-Rad51 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
See below the WB image, lot number and protocol we used for testis using anti-Rad51 Rabbit Monoclonal antibody M00088. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
I was wanting to use your anti-Rad51 Rabbit Monoclonal antibody for IP for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
As indicated on the product datasheet, M00088 anti-Rad51 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-22
Question
My lab would like to test anti-Rad51 Rabbit Monoclonal antibody M00088 on human testis for research purposes, then I may be interested in using anti-Rad51 Rabbit Monoclonal antibody M00088 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The products we sell, including anti-Rad51 Rabbit Monoclonal antibody M00088, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-24
Question
Is this M00088 anti-Rad51 Rabbit Monoclonal antibody reactive to the isotypes of RAD51?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
The immunogen of M00088 anti-Rad51 Rabbit Monoclonal antibody is A synthesized peptide derived from human Rad51. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-07
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Rad51 Rabbit Monoclonal antibody M00088. Let me know if you need anything else.
S. Yang
Verified customer
Asked: 2018-10-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-03
Question
We have been able to see staining in human placenta. Any tips? Is anti-Rad51 Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
According to literature placenta does express RAD51. According to Uniprot.org, RAD51 is expressed in embryo, testis, brain, placenta, among other tissues. Regarding which tissues have RAD51 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Testis, Pubmed ID: 8479919
Boster Scientific Support
Answered: 2018-01-29
Question
Would anti-Rad51 Rabbit Monoclonal antibody M00088 work for IP with testis?
L. Mangal
Verified customer
Asked: 2018-01-01
Answer
According to the expression profile of testis, RAD51 is highly expressed in testis. So, it is likely that anti-Rad51 Rabbit Monoclonal antibody M00088 will work for IP with testis.
Boster Scientific Support
Answered: 2018-01-01
Question
My question regards using your anti-Rad51 Rabbit Monoclonal antibody for double-strand break repair via homologous recombination studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
Thanks for your inquiry. This M00088 anti-Rad51 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-06-22
Question
We are currently using anti-Rad51 Rabbit Monoclonal antibody M00088 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
A. Lewis
Verified customer
Asked: 2017-02-08
Answer
The anti-Rad51 Rabbit Monoclonal antibody (M00088) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-08
Question
Our team were satisfied with the WB result of your anti-Rad51 Rabbit Monoclonal antibody. However we have been able to see positive staining in embryo nucleus using this antibody. Is that expected? Could you tell me where is RAD51 supposed to be expressed?
W. Krishna
Verified customer
Asked: 2016-09-06
Answer
From literature, embryo does express RAD51. Generally RAD51 expresses in nucleus. Regarding which tissues have RAD51 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Testis, Pubmed ID: 8479919
Boster Scientific Support
Answered: 2016-09-06