Anti-Rad51 Rabbit Monoclonal Antibody

RAD51 antibody

Boster Bio Anti-Rad51 Rabbit Monoclonal Antibody catalog # M00088. Tested in WB, Flow Cytometry, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00088
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, WB

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Product Name

Anti-Rad51 Rabbit Monoclonal Antibody

View all RAD51 Antibodies

SKU/Catalog Number

M00088

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Rad51 Rabbit Monoclonal Antibody catalog # M00088. Tested in WB, Flow Cytometry, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Rad51 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00088)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CAE-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Rad51

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00088 is reactive to RAD51 in Human, Mouse, Rat

Applications

M00088 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

36.966kDa

Background of RAD51

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For RAD51 (Source: Uniprot.org, NCBI)

Gene Name

RAD51

Full Name

DNA repair protein RAD51 homolog 1

Weight

36.966kDa

Superfamily

RecA family

Alternative Names

BRCC5; HRAD51; HsRad51; HsT16930; RAD51 (S. cerevisiae) homolog (E coli RecA homolog); RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RAD51 homolog A; Rad51; RAD51ABRCA1/BRCA2-containing complex, subunit 5; RAD51L3; RecA, E. coli, homolog of; RECADNA repair protein RAD51 homolog 1; RecA-like protein; recombination protein A RAD51 BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2A, RECA, RAD51 RAD51 recombinase DNA repair protein RAD51 homolog 1|BRCA1/BRCA2-containing complex, subunit 5|RAD51 homolog A|RecA, E. coli, homolog of|RecA-like protein|recombination protein A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RAD51, check out the RAD51 Infographic

RAD51 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAD51: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-Rad51 Rabbit Monoclonal Antibody

Question

Would M00088 anti-Rad51 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

It shows on the product datasheet, M00088 anti-Rad51 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-16

Question

We ordered your anti-Rad51 Rabbit Monoclonal antibody for IP on embryo in a previous experiment. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining embryo as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

I have checked the website and datasheets of our anti-Rad51 Rabbit Monoclonal antibody and it appears that M00088 has been tested on mouse in both IP and WB. Thus M00088 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-14

Question

I see that the anti-Rad51 Rabbit Monoclonal antibody M00088 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-28

Question

Is a blocking peptide available for product anti-Rad51 Rabbit Monoclonal antibody (M00088)?

K. Banerjee

Verified customer

Asked: 2020-01-15

Answer

We do provide the blocking peptide for product anti-Rad51 Rabbit Monoclonal antibody (M00088). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-15

Question

Is there a BSA free version of anti-Rad51 Rabbit Monoclonal antibody M00088 available?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rad51 Rabbit Monoclonal antibody M00088 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-19

Question

Can you help my question with product M00088, anti-Rad51 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00088 anti-Rad51 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-18

Question

See below the WB image, lot number and protocol we used for testis using anti-Rad51 Rabbit Monoclonal antibody M00088. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-14

Question

I was wanting to use your anti-Rad51 Rabbit Monoclonal antibody for IP for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

As indicated on the product datasheet, M00088 anti-Rad51 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-22

Question

My lab would like to test anti-Rad51 Rabbit Monoclonal antibody M00088 on human testis for research purposes, then I may be interested in using anti-Rad51 Rabbit Monoclonal antibody M00088 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

The products we sell, including anti-Rad51 Rabbit Monoclonal antibody M00088, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-24

Question

Is this M00088 anti-Rad51 Rabbit Monoclonal antibody reactive to the isotypes of RAD51?

Verified Customer

Verified customer

Asked: 2018-11-07

Answer

The immunogen of M00088 anti-Rad51 Rabbit Monoclonal antibody is A synthesized peptide derived from human Rad51. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-11-07

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Rad51 Rabbit Monoclonal antibody M00088. Let me know if you need anything else.

S. Yang

Verified customer

Asked: 2018-10-03

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-03

Question

We have been able to see staining in human placenta. Any tips? Is anti-Rad51 Rabbit Monoclonal antibody supposed to stain placenta positively?

Verified Customer

Verified customer

Asked: 2018-01-29

Answer

According to literature placenta does express RAD51. According to Uniprot.org, RAD51 is expressed in embryo, testis, brain, placenta, among other tissues. Regarding which tissues have RAD51 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Testis, Pubmed ID: 8479919

Boster Scientific Support

Answered: 2018-01-29

Question

Would anti-Rad51 Rabbit Monoclonal antibody M00088 work for IP with testis?

L. Mangal

Verified customer

Asked: 2018-01-01

Answer

According to the expression profile of testis, RAD51 is highly expressed in testis. So, it is likely that anti-Rad51 Rabbit Monoclonal antibody M00088 will work for IP with testis.

Boster Scientific Support

Answered: 2018-01-01

Question

My question regards using your anti-Rad51 Rabbit Monoclonal antibody for double-strand break repair via homologous recombination studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-06-22

Answer

Thanks for your inquiry. This M00088 anti-Rad51 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-06-22

Question

We are currently using anti-Rad51 Rabbit Monoclonal antibody M00088 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

A. Lewis

Verified customer

Asked: 2017-02-08

Answer

The anti-Rad51 Rabbit Monoclonal antibody (M00088) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-02-08

Question

Our team were satisfied with the WB result of your anti-Rad51 Rabbit Monoclonal antibody. However we have been able to see positive staining in embryo nucleus using this antibody. Is that expected? Could you tell me where is RAD51 supposed to be expressed?

W. Krishna

Verified customer

Asked: 2016-09-06

Answer

From literature, embryo does express RAD51. Generally RAD51 expresses in nucleus. Regarding which tissues have RAD51 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Testis, Pubmed ID: 8479919

Boster Scientific Support

Answered: 2016-09-06

Order DetailsPrice
M00088

100μL

$370
M00088-30ul

30μL sample

$99
M00088-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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