Anti-IFN gamma Antibody

Boster Bio Anti-IFN gamma Antibody catalog # RP1001. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. Cited in 6 publication(s).

Product Info Summary

SKU: RP1001
Size: 100μg/vial
Reactive Species: Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-IFN gamma Antibody

See all IFN-gamma products

SKU/Catalog Number

RP1001

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IFN gamma Antibody catalog # RP1001. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IFN gamma Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1001)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived rat IFN gamma recombinant protein (Position: 23-156).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1001 is reactive to Ifng in Rat

Applications

RP1001 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
ELISA , 0.1-0.5μg/ml, Rat, -
Western blot, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Ifng (Source: Uniprot.Org, NCBI)

Uniprot ID

P01581

Gene ID

25712

Gene Name

Ifng

Full Name

Interferon gamma

Weight

17.918kDa

Superfamily

type II (or gamma) interferon family

Alternative Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma Ifng|IFNG2, If2f|interferon gamma|interferon gamma|IFN-gamma|interferon 2f

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ifng, check out the Ifng Infographic

Ifng infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ifng: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1001 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li Q,Sun J,Cao Y,Liu B,Li L,Mohammadtursun N,Zhang H,Dong J,Wu J.Bu-Shen-Fang-Chuan formula attenuates T-lymphocytes recruitment in the lung of rats with COPD through suppressing CXCL9/CXCL10/CXCL11-CXCR3 axis.Biomed Pharmacother.2020 Mar;123:109735.doi:1
Species: Rat
RP1001 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA

Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface

Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation

Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion

Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

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Find answers in Q&As, reviews.

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16 Customer Q&As for Anti-IFN gamma Antibody

Question

Will anti-IFN gamma antibody RP1001 work on goat WB with blood?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

Our lab technicians have not validated anti-IFN gamma antibody RP1001 on goat. You can run a BLAST between goat and the immunogen sequence of anti-IFN gamma antibody RP1001 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat blood in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-20

Question

We are currently using anti-IFN gamma antibody RP1001 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The anti-IFN gamma antibody (RP1001) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-31

Question

We ordered your anti-IFN gamma antibody for ELISA on leukocyte last year. I am using rat, and We are going to use the antibody for IHC next. I am looking for examining leukocyte as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-01-21

Answer

I have checked the website and datasheets of our anti-IFN gamma antibody and it appears that RP1001 has been tested on rat in both ELISA and IHC. Thus RP1001 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-21

Question

Would anti-IFN gamma antibody RP1001 work for WB with blood?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

According to the expression profile of blood, IFNG is highly expressed in blood. So, it is likely that anti-IFN gamma antibody RP1001 will work for WB with blood.

Boster Scientific Support

Answered: 2019-10-01

Question

Would RP1001 anti-IFN gamma antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

It shows on the product datasheet, RP1001 anti-IFN gamma antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-19

Question

I see that the anti-IFN gamma antibody RP1001 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-25

Question

Is this RP1001 anti-IFN gamma antibody reactive to the isotypes of IFNG?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

The immunogen of RP1001 anti-IFN gamma antibody is E. coli-derived rat IFN gamma recombinant protein(Position: 23-156). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-14

Question

We have observed staining in rat blood. What should we do? Is anti-IFN gamma antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2019-01-30

Answer

From what I have seen in literature blood does express IFNG. From what I have seen in Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-01-30

Question

See attached the WB image, lot number and protocol we used for blood using anti-IFN gamma antibody RP1001. Please let me know if you require anything else.

A. Anderson

Verified customer

Asked: 2018-12-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-03

Question

Is a blocking peptide available for product anti-IFN gamma antibody (RP1001)?

Verified Customer

Verified customer

Asked: 2017-11-16

Answer

We do provide the blocking peptide for product anti-IFN gamma antibody (RP1001). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-16

Question

I am looking for to test anti-IFN gamma antibody RP1001 on rat blood for research purposes, then I may be interested in using anti-IFN gamma antibody RP1001 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

G. Jha

Verified customer

Asked: 2017-08-22

Answer

The products we sell, including anti-IFN gamma antibody RP1001, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-08-22

Question

Is there a BSA free version of anti-IFN gamma antibody RP1001 available?

Verified Customer

Verified customer

Asked: 2017-07-20

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IFN gamma antibody RP1001 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-07-20

Question

I was wanting to use your anti-IFN gamma antibody for WB for rat blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat blood identification?

M. Patel

Verified customer

Asked: 2017-02-28

Answer

As indicated on the product datasheet, RP1001 anti-IFN gamma antibody has been validated for ELISA, IHC, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-02-28

Question

Our team were satisfied with the WB result of your anti-IFN gamma antibody. However we have observed positive staining in leukocyte secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?

K. Miller

Verified customer

Asked: 2016-12-01

Answer

From what I have seen in literature, leukocyte does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2016-12-01

Question

My question regarding product RP1001, anti-IFN gamma antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Rodriguez

Verified customer

Asked: 2014-11-03

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1001 anti-IFN gamma antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-11-03

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-IFN gamma antibody RP1001. Let me know if you need anything else.

A. Krishna

Verified customer

Asked: 2013-06-25

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-06-25

Conjugation

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RP1001
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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