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- Table of Contents
Ifng antibody (RP1001) performs well in ELISA, IHC, WB and shows reactivity with Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Figure. Western blot analysis of IFN gamma using anti-IFN gamma antibody (RP1001).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane : Recombinant Rat IFN gamma Protein 0.5ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN gamma antigen affinity purified polyclonal antibody (Catalog # RP1001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFN gamma at approximately 22KD. The expected band size for IFN gamma is at 22KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane : Recombinant Rat IFN gamma Protein 0.5ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN gamma antigen affinity purified polyclonal antibody (Catalog # RP1001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFN gamma at approximately 22KD. The expected band size for IFN gamma is at 22KD.
Designing a Western blot for IFNG? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the IFNG Western Blot GuideBundle Save
Anti-IFN gamma Antibody Picoband®
Ifng antibody
| Reactive Species | Host | Application |
|---|---|---|
| Rat | Rabbit | ELISA, IHC, WB |
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Rat
ELISA
0.1-0.5μg/ml
-
Western blot
0.1-0.5μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IFN gamma Antibody Picoband®
View all IFNG Antibodies
View all IFNG Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat IFN gamma recombinant protein (Position: 23-156).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
17.9 kDa
Background of Ifng
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Cite This Product
Anti-IFN gamma Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1001)
Publications
Application:
Species:
Li Q,Sun J,Cao Y,Liu B,Li L,Mohammadtursun N,Zhang H,Dong J,Wu J.Bu-Shen-Fang-Chuan formula attenuates T-lymphocytes recruitment in the lung of rats with COPD through suppressing CXCL9/CXCL10/CXCL11-CXCR3 axis.Biomed Pharmacother.2020 Mar;123:109735.doi:1
Species: Rat
PMID: 31864210
Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface
PMID: 28902929
Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation
PMID: 26885268
Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion
PMID: 26222793
Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques
PMID: 24798997
Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.
PMID: 25483698
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Product Promise
We promise Anti-IFN gamma Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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RP1001
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 25, 2026