Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M00393-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Interferon gamma IFNG Rabbit Monoclonal Antibody
SKU/Catalog Number
M00393-1
BM4939 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Interferon gamma IFNG Rabbit Monoclonal Antibody catalog # M00393-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interferon gamma IFNG Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00393-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOFD-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Interferon gamma
Reactive Species
M00393-1 is reactive to IFNG in Human, Mouse, Rat
Observed Molecular Weight
17 kDa
Calculated molecular weight
19.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00393-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Interferon gamma expression in Jurkat cell lysate.
Click image to see more details
Western blot analysis of IFNG using anti-IFNG antibody (M00393-1).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: recombinant human IFNG protein.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified monoclonal antibody (M00393-1) at 1:500 overnight at 4°C, then washed with TBS-10%%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IFNG at approximately 17 kDa. The expected band size for IFNG is at 17 kDa.
Click image to see more details
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
Specific Publications For Anti-Interferon gamma IFNG Rabbit Monoclonal Antibody (M00393-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Interferon gamma IFNG Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Interferon gamma IFNG Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-Interferon gamma IFNG Rabbit Monoclonal Antibody
Question
Our lab used your anti-Interferon gamma Rabbit Monoclonal antibody for WB on blood last year. I am using human, and We are going to use the antibody for IF next. I am interested in examining blood as well as leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
I viewed the website and datasheets of our anti-Interferon gamma Rabbit Monoclonal antibody and I see that M00393-1 has been tested on human in both WB and IF. Thus M00393-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-07
Question
My boss were content with the WB result of your anti-Interferon gamma Rabbit Monoclonal antibody. However we have seen positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
From what I have seen in literature, blood does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-28
Question
We have observed staining in rat blood. What should we do? Is anti-Interferon gamma Rabbit Monoclonal antibody supposed to stain blood positively?
T. Collins
Verified customer
Asked: 2020-01-29
Answer
From literature blood does express IFNG. From Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-29
Question
Is this M00393-1 anti-Interferon gamma Rabbit Monoclonal antibody reactive to the isotypes of IFNG?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
The immunogen of M00393-1 anti-Interferon gamma Rabbit Monoclonal antibody is A synthesized peptide derived from human Interferon gamma. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-16
Question
I have attached the WB image, lot number and protocol we used for blood using anti-Interferon gamma Rabbit Monoclonal antibody M00393-1. Please let me know if you require anything else.
P. Evans
Verified customer
Asked: 2019-09-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-06
Question
Do you have a BSA free version of anti-Interferon gamma Rabbit Monoclonal antibody M00393-1 available?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Interferon gamma Rabbit Monoclonal antibody M00393-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-29
Question
Does anti-Interferon gamma Rabbit Monoclonal antibody M00393-1 work for Flow Cytometry with blood?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
According to the expression profile of blood, IFNG is highly expressed in blood. So, it is likely that anti-Interferon gamma Rabbit Monoclonal antibody M00393-1 will work for Flow Cytometry with blood.
Boster Scientific Support
Answered: 2019-06-10
Question
Would M00393-1 anti-Interferon gamma Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
You can see on the product datasheet, M00393-1 anti-Interferon gamma Rabbit Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-04
Question
I was wanting to use your anti-Interferon gamma Rabbit Monoclonal antibody for Flow Cytometry for mouse blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse blood identification?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
As indicated on the product datasheet, M00393-1 anti-Interferon gamma Rabbit Monoclonal antibody has been validated for Flow Cytometry, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-04
Question
I am interested in using your anti-Interferon gamma Rabbit Monoclonal antibody for regulation of insulin secretion studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
Thank you for your inquiry. This M00393-1 anti-Interferon gamma Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for Flow Cytometry, IF, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-01-30
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Interferon gamma Rabbit Monoclonal antibody M00393-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-08
Question
I have a question about product M00393-1, anti-Interferon gamma Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00393-1 anti-Interferon gamma Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-09
Question
Is a blocking peptide available for product anti-Interferon gamma Rabbit Monoclonal antibody (M00393-1)?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
We do provide the blocking peptide for product anti-Interferon gamma Rabbit Monoclonal antibody (M00393-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-10-02
Question
We are currently using anti-Interferon gamma Rabbit Monoclonal antibody M00393-1 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
The anti-Interferon gamma Rabbit Monoclonal antibody (M00393-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-20
Question
My question regards to test anti-Interferon gamma Rabbit Monoclonal antibody M00393-1 on mouse blood for research purposes, then I may be interested in using anti-Interferon gamma Rabbit Monoclonal antibody M00393-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Huang
Verified customer
Asked: 2016-10-11
Answer
The products we sell, including anti-Interferon gamma Rabbit Monoclonal antibody M00393-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-10-11
Question
I see that the anti-Interferon gamma Rabbit Monoclonal antibody M00393-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
B. Thomas
Verified customer
Asked: 2015-12-22
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-12-22
