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- Table of Contents
RBM15B antibody (A11484-1) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of Daudi cells using anti-RBM15B antibody (A11484-1).
Overlay histogram showing Daudi cells stained with A11484-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM15B Antibody (A11484-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Daudi cells stained with A11484-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM15B Antibody (A11484-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-RBM15B Antibody Picoband®
RBM15B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
RBM15B antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human MOLT-4 whole cell, human RT4 whole cell, rat C6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
RBM15B antibody was positively detected by IHC in:
human breast cancer tissue, human esophageal squamous carcinoma tissue, human gastric adenocarcinoma tissue, human liver cancer tissue, human lung squamous cell carcinoma tissue, human ovary serous carcinoma tissue, human rectum adenocarcinoma tissue, human undifferentiated carcinoma of pancreas tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
RBM15B antibody was positively detected by IF in:
human intestinal cancer tissue, human ovarian cancer tissue
RBM15B antibody was positively detected by FC in:
Daudi cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Recommend
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-RBM15B Antibody Picoband®
View all RBM15B Antibodies
View all RBM15B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RBM15B recombinant protein (Position: K164-A890).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
110 kDa
Calculated Molecular Weight
97.2 kDa
Background of RBM15B
Members of the SPEN (Split-end) family of proteins, including RBM15B, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome component.
Cite This Product
Anti-RBM15B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11484-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
98%
High Homology
Cow
97%
High Homology
Mouse
97%
High Homology
Sheep
97%
High Homology
Rat
97%
High Homology
Drosophila
36%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for RBM15B antibody
Immunohistochemistry (IHC) protocol for RBM15B antibody
Immunofluorescence (IF) protocol for RBM15B antibody
Flow Cytometry (FC) protocol for RBM15B antibody
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Product Promise
We promise Anti-RBM15B Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A11484-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026