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Product Info Summary
| SKU: | A03075 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, IHC |
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Product info
Product Name
Anti-Reg I alpha REG1A Monoclonal Antibody
SKU/Catalog Number
A03075
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Reg I alpha REG1A Monoclonal Antibody catalog # A03075. Tested in IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Reg I alpha REG1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03075)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide, 0.5% stabilizing protein, 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
1A4
Isotype
IgG1
Immunogen
Purified recombinant fragment of human Reg Iα fused with hIgGFc tag expressed in HEK293 cell line.
Reactive Species
A03075 is reactive to REG1A in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
18.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03075 is guaranteed for ELISA, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Confocal immunofluorescence analysis of PC12 cells using Reg Iα Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
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Immunohistochemistry analysis of paraffin-embedded human Pancreas tissues with AEC staining using Reg Iα Monoclonal Antibody.
Specific Publications For Anti-Reg I alpha REG1A Monoclonal Antibody (A03075)
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Customer Q&As
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3 Customer Q&As for Anti-Reg I alpha REG1A Monoclonal Antibody
Question
Would anti-Reg I alpha Monoclonal antibody A03075 work on zebrafish IHC with islet of langerhans?
C. Banerjee
Verified customer
Asked: 2019-10-25
Answer
Our lab technicians have not validated anti-Reg I alpha Monoclonal antibody A03075 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Reg I alpha Monoclonal antibody A03075 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish islet of langerhans in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
We are currently using anti-Reg I alpha Monoclonal antibody A03075 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The anti-Reg I alpha Monoclonal antibody (A03075) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-30
Question
Will A03075 anti-Reg I alpha Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Yang
Verified customer
Asked: 2018-08-08
Answer
As indicated on the product datasheet, A03075 anti-Reg I alpha Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-08
