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Product Info Summary
| SKU: | A03075 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, IHC-P |
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Product info
Product Name
Anti-Reg I alpha REG1A Monoclonal Antibody
SKU/Catalog Number
A03075
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Reg I alpha REG1A Monoclonal Antibody catalog # A03075. Tested in IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Reg I alpha REG1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03075)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.
Clonality
Monoclonal
Clone Number
1A4
Isotype
IgG1
Immunogen
Purified recombinant fragment of human Reg Ialpha fused with hIgGFc tag expressed in HEK293 cell line.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03075 is reactive to REG1A in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
18731 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03075 is guaranteed for ELISA, IF, IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Positive Control
Validation Images & Assay Conditions
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Pancreas tissues with AEC staining using Reg Ialpha Monoclonal antibody.
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Confocal Immunofluorescence (IF) analysis of PC12 cells using Reg Ialpha Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Protein Target Info & Infographic
Gene/Protein Information For REG1A (Source: Uniprot.org, NCBI)
Gene Name
REG1A
Full Name
Lithostathine-1-alpha
Weight
18731 MW
Alternative Names
ICRF; Islet cells regeneration factor; Islet of Langerhans regenerating protein; lithostathine-1-alpha; P19; pancreatic stone protein, secretory; Pancreatic thread protein; protein-X; PSP; PSPS; PSPS1; PTP; REG; Reg1A; REG-1-alpha; Regenerating islet-derived protein 1-alpha; Regenerating protein I alpha REG1A ICRF, P19, PSP, PSPS, PSPS1, PTP, REG regenerating family member 1 alpha lithostathine-1-alpha|REG-1-alpha|islet cells regeneration factor|islet of langerhans regenerating protein|pancreatic stone protein, secretory|pancreatic thread protein|protein-X|regenerating islet-derived protein 1-alpha|regenerating protein I alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on REG1A, check out the REG1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for REG1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Reg I alpha REG1A Monoclonal Antibody (A03075)
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No publications found for A03075
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Reg I alpha REG1A Monoclonal Antibody
Question
Would anti-Reg I alpha Monoclonal antibody A03075 work on zebrafish IHC with islet of langerhans?
C. Banerjee
Verified customer
Asked: 2019-10-25
Answer
Our lab technicians have not validated anti-Reg I alpha Monoclonal antibody A03075 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Reg I alpha Monoclonal antibody A03075 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish islet of langerhans in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
We are currently using anti-Reg I alpha Monoclonal antibody A03075 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The anti-Reg I alpha Monoclonal antibody (A03075) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-30
Question
Will A03075 anti-Reg I alpha Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Yang
Verified customer
Asked: 2018-08-08
Answer
As indicated on the product datasheet, A03075 anti-Reg I alpha Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-08
