Anti-RET Antibody Picoband™

RET antibody

Boster Bio Anti-RET Antibody Picoband™ catalog # A00293-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00293-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-RET Antibody Picoband™

View all RET Antibodies

SKU/Catalog Number

A00293-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RET Antibody Picoband™ catalog # A00293-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RET Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00293-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human RET recombinant protein (Position: L29-V304).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00293-1 is reactive to RET in Human, Mouse, Rat

Applications

A00293-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of RET

The RET proto-oncogene encodes a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor (GDNF) family of extracellular signalling molecules. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For RET (Source: Uniprot.Org, NCBI)

Uniprot ID

P07949

Gene ID

5979

Gene Name

RET

Full Name

Proto-oncogene tyrosine-protein kinase receptor Ret

Weight

124.319kDa

Superfamily

protein kinase superfamily

Alternative Names

CDHF12MTC1; CDHR16; EC 2.7.10; Hirschsprung disease 1; Hirschsprung disease); MEN2A; MEN2B; multiple endocrine neoplasia and medullary thyroid carcinoma 1; ret proto-oncogene; RET transforming sequence; Ret RET CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC-ELE1, RET ret proto-oncogene proto-oncogene tyrosine-protein kinase receptor Ret|RET receptor tyrosine kinase|cadherin family member 12|cadherin-related family member 16|proto-oncogene c-Ret|rearranged during transfection|ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RET, check out the RET Infographic

RET infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RET: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-RET Antibody Picoband™

Question

My colleagues were satisfied with the WB result of your anti-RET antibody. However we have seen positive staining in prostate cell membrane using this antibody. Is that expected? Could you tell me where is RET supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

Based on literature, prostate does express RET. Generally RET expresses in cell membrane. Regarding which tissues have RET expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7633441
Brain, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 2734021
Leukocyte, Pubmed ID: 7581377
Peripheral blood leukocyte, Pubmed ID: 9360560
Prostate, Pubmed ID: 14702039
Thyroid papillary carcinoma, Pubmed ID: 2406025

Boster Scientific Support

Answered: 2020-03-03

Question

We ordered your anti-RET antibody for IHC-P on brain a few months ago. I am using rat, and I plan to use the antibody for ELISA next. I would like examining brain as well as blood in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

I took a look at the website and datasheets of our anti-RET antibody and I see that A00293-1 has been tested on rat in both IHC-P and ELISA. Thus A00293-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-30

Question

I was wanting to use using your anti-RET antibody for lymphocyte migration into lymphoid organs studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.

S. Collins

Verified customer

Asked: 2018-09-12

Answer

We appreciate your inquiry. This A00293-1 anti-RET antibody is tested on mouse brain. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-09-12

Question

We have seen staining in rat dorsal root ganglion. Any tips? Is anti-RET antibody supposed to stain dorsal root ganglion positively?

Verified Customer

Verified customer

Asked: 2017-08-04

Answer

From what I have seen in literature dorsal root ganglion does express RET. From what I have seen in Uniprot.org, RET is expressed in dorsal root ganglion, prostate, brain, fibroblast, thyroid papillary carcinoma, blood, leukocyte, peripheral blood leukocyte, among other tissues. Regarding which tissues have RET expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7633441
Brain, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 2734021
Leukocyte, Pubmed ID: 7581377
Peripheral blood leukocyte, Pubmed ID: 9360560
Prostate, Pubmed ID: 14702039
Thyroid papillary carcinoma, Pubmed ID: 2406025

Boster Scientific Support

Answered: 2017-08-04

Question

We are currently using anti-RET antibody A00293-1 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-14

Answer

The anti-RET antibody (A00293-1) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-14

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00293-1

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A00293-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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