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- Table of Contents
RHOA antibody (M00207-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Immunofluorescent analysis of Hela cells, using Rho A + B + C Antibody .
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Anti-Rho A + B + C Rabbit Monoclonal Antibody
RHOA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
RHOA antibody was positively detected by WB in:
human HUVEC whole cell, human THP-1 whole cell, human Hela whole cell, human U251 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue, human 293T whole cell, human SH-SY5Y whole cell, human C6 whole cell, human neuro-2α whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
RHOA antibody was positively detected by IHC in:
human lung carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
RHOA antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-Rho A + B + C Rabbit Monoclonal Antibody
View all RHOA Antibodies
View all RHOA Antibodies
Form
Liquid
Uniprot
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DGF-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rho A + B + C
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
22 kDa
Cite This Product
Anti-Rho A + B + C Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00207-1)
Protocols
Western Blot protocol for RHOA antibody
Immunohistochemistry (IHC) protocol for RHOA antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for RHOA antibody
Publications
Application:
Species:
Longitudinal genomic alternations and clonal dynamics analysis of primary malignant melanoma of the esophagus
Published: 2022-06-01
Journal: NEOPLASIA
Application: IHC
Impact Factor: 6.22
Species: Human
PMID: 35661532
Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress
Published: 2018-04-04
Journal: Gastroenterology Research and Practice
Application: IHC,WB
Impact Factor: 1.86
Species: Rat
PMID: 29849603
Review
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Submit A Review2 Reviews For Anti-Rho A + B + C Rabbit Monoclonal Antibody
This antibody is highly specific and efficient, suitable for detecting RHOA/B/C protein in rat colon by Western blot, with only minimal non-specific bands.
| SKU | M00207-1 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | Rho A + B + C Rabbit Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:5000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows the Western blot results of the target protein RHOA/B/C and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and distinct, and the experimental results are satisfactory. |
Shiyu Zhang, LUTCM
Verified customer
Submitted 2026-01-06
The target band of this antibody is clear and at the correct position, with minimal nonspecific bands, and the results are good.
| SKU | M00207-1 |
|---|---|
| Application | Western Blot |
| Sample | huaman 293 cells |
| Sample Processing Description | RHOB detection was performed using total protein extracted from normal 293 cells and 293 cells with RHOB overexpression. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | ATF4 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2500, overnight at 4 ℃ |
| Secondary Antibody | HRP Goat Anti-Rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | We generated a stable RHOB overexpression cell line using 293 cells. The results show that the RHOB expression level in the transfected samples is significantly higher than in the normal 293 cells, indicating successful transfection. |
Jie Zhang, Zhejiang A&F University
Verified customer
Submitted 2025-12-04
Product Promise
We promise Anti-Rho A + B + C Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00207-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 17, 2026