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CDK4 antibody (PB9535) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 21 Publications

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pb9535 cdk4 primary antibodies if testing 5

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IF analysis of CDK4 using anti-CDK4 antibody (PB9535).
CDK4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDK4 Antibody (PB9535) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Designing a Western blot or IHC for CDK4? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.

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Anti-Cdk4 Antibody Picoband®

CDK4 antibody

21 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IF, IHC, WB

Tested Applications

CDK4 antibody was positively detected by WB in:

human Hela whole cell, human K562 whole cell, rat PC-12 whole cell, mosue NIH/3T3 whole cell, mosue RAW2647 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CDK4 antibody was positively detected by IHC in:

mouse intestine tissue, rat intestine tissue, human lung cancer tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CDK4 antibody was positively detected by IF in:

human colon cancer tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Dog

Predicted

Bovine

Predicted

Sheep

Predicted

Rabbit

Predicted

Product Information

Product Name

Anti-Cdk4 Antibody Picoband®
View all CDK4 Antibodies

Form

Lyophilized

Uniprot

P11802

Gene ID

1019

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Cdk4 recombinant protein (Position: G201-E303). Human Cdk4 shares 94.2% amino acid (aa) sequence identity with both mouse and rat Cdk4.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

34 kDa

Calculated Molecular Weight

33.7 kDa

Background of CDK4

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. And CDK4 expression and activity are required for cytokine responsiveness in T cells.

Cite This Product

Anti-Cdk4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9535)

Protocols

Western Blot protocol for CDK4 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CDK4 antibody

Review Protocol

Immunofluorescence (IF) protocol for CDK4 antibody

Review Protocol

Publications

Association Between CDK4/CDK6 Expression and Prognosis in Patients with Adenocarcinoma of Gastroesophageal Junction

Published: 2025-06-17

Journal: DIGESTIVE DISEASES AND SCIENCES

Application: IHC

Impact Factor: 2.50

Species: Human

PMID: 40526319

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Anti-Cdk4 Antibody Picoband®

CDK4 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Association Between CDK4/CDK6 Expression and Prognosis in Patients with Adenocarcinoma of Gastroesophageal Junction

Icariside I reduces breast cancer proliferation, apoptosis, invasion, and metastasis probably through inhibiting IL-6/STAT3 signaling pathway

GO nanosheets inhibit the proliferation of hPDLCs by covering the membrane to block the EGFR-AKT signaling pathway

Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway

Combined Anti-Cancer Effects of Platycodin D and Sorafenib on Androgen-Independent and PTEN-Deficient Prostate Cancer

Chemopreventive Role of Apigenin against the Synergistic Carcinogenesis of Human Papillomavirus and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone

Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulating matrix metalloproteinase 1 expression through inhibition of the ERK1/2/NF-κB signalling pathway

Targeted p21 activation by a new double stranded RNA suppresses human prostate cancer cells growth and metastasis

Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles

JNK/STAT signalling pathway is involved in fluoride-induced follicular developmental dysplasia in female mice

Wall shear stress promotes intimal hyperplasia through the paracrine H2O2-mediated NOX-AKT-SVV axis

OAMDP, a novel podophyllotoxin derivative, induces apoptosis, cell cycle arrest and autophagy in hepatoma HepG2 cells

Effects of miR-1236-3p and miR-370-5p on activation of p21 in various tumors and its inhibition on the growth of lung cancer cells

Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 oncogene

Melittin Restores PTEN Expression by Down-Regulating HDAC2 in Human Hepatocelluar Carcinoma HepG2 Cells

Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins

The effects of artemisinin on the proliferation and apoptosis of vascular smooth muscle cells of rats

Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric Cancer Cells

Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo

Dynamic changes of cell cycle elements in the ischemic brain after bone marrow stromal cells transplantation in rats

Immunoregulatory and Anti-Tumor Effects of Polysaccharopeptide and Astragalus polysaccharides on Tumor-Bearing Mice

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