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Product Info Summary
| SKU: | M04186 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-ROS1 Mouse Monoclonal Antibody [Clone ID: OTI1F3]
SKU/Catalog Number
M04186
Size
100 µl
Description
Boster Bio ROS1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3). Catalog# M04186. Tested in IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ROS1 Mouse Monoclonal Antibody [Clone ID: OTI1F3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04186)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1F3
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 2126-2347 of human ROS1 (NP_002935) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04186 is reactive to ROS1 in Human
Reconstitution
Calculated molecular weight
263.915kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04186 is guaranteed for IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROS1.
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Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ROS1 monoclonal antibody (1:500).
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Immunohistochemical staining of paraffin-embedded HCC78 (left) and HeLa (right) xenograft using M04186 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (M04186; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (M04186; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (M04186; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunocytochemistry staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (M04186) (Left). The right is HELA cells as negative control (1:2000).
Protein Target Info & Infographic
Gene/Protein Information For ROS1 (Source: Uniprot.org, NCBI)
Gene Name
ROS1
Full Name
Proto-oncogene tyrosine-protein kinase ROS
Weight
263.915kDa
Superfamily
protein kinase superfamily
Alternative Names
proto-oncogene tyrosine-protein kinase ROS; c-ros oncogene 1, receptor tyrosine kinase; c-ros-1; MCF3; ROS ROS1 MCF3, ROS, c-ros-1 ROS proto-oncogene 1, receptor tyrosine kinase proto-oncogene tyrosine-protein kinase ROS|ROS proto-oncogene 1 , receptor tyrosine kinase|c-ros oncogene 1 , receptor tyrosine kinase|proto-oncogene c-Ros-1|transmembrane tyrosine-specific protein kinase|v-ros avian UR2 sarcoma virus oncogene homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ROS1, check out the ROS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ROS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ROS1 Mouse Monoclonal Antibody [Clone ID: OTI1F3] (M04186)
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