Product Info Summary
| SKU: | M04186 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-ROS1 Mouse Monoclonal Antibody [Clone ID: OTI1F3]
SKU/Catalog Number
M04186
Size
100 µl
Description
Boster Bio ROS1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3). Catalog# M04186. Tested in IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ROS1 Mouse Monoclonal Antibody [Clone ID: OTI1F3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04186)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1F3
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 2126-2347 of human ROS1 (NP_002935) produced in E.coli.
Reactive Species
M04186 is reactive to ROS1 in Human
Calculated molecular weight
263.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04186 is guaranteed for IF, IHC, WB Boster Guarantee
Recommend Dilution
WB 1:2000
IHC 1:5000
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROS1.
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Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ROS1 monoclonal antibody (1:500).
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Immunohistochemical staining of paraffin-embedded HCC78 (left) and HeLa (right) xenograft using M04186 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (M04186; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (M04186; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of ROS1 positive Human lung tissue using anti-ROS1 mouse monoclonal antibody. (M04186; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunocytochemistry staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (M04186) (Left). The right is HELA cells as negative control (1:2000).
Specific Publications For Anti-ROS1 Mouse Monoclonal Antibody [Clone ID: OTI1F3] (M04186)
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