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Product Info Summary
| SKU: | P01029-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-POLR2A (S5) Rabbit Monoclonal Antibody
SKU/Catalog Number
P01029-2
BM5116 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-POLR2A (S5) Rabbit Monoclonal Antibody catalog # P01029-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-POLR2A (S5) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01029-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABCE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human POLR2A
Reactive Species
P01029-2 is reactive to POLR2A in Human, Mouse, Rat
Observed Molecular Weight
270 kDa
Calculated molecular weight
217.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01029-2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:40
Positive Control
WB: HeLa cell
IHC: human spleen tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-POLR2A (S5) expression in (1) HeLa cell lysate treated with Lambda Phosphatase; (2) HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded human spleen, using Phospho-POLR2A (S5) Antibody.
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Immunofluorescent analysis of Hela cells, using Phospho-POLR2A (S5) Antibody .
Specific Publications For Anti-Phospho-POLR2A (S5) Rabbit Monoclonal Antibody (P01029-2)
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