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Product Info Summary
| SKU: | A02049-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RuvB-like 1 RUVBL1 Antibody
SKU/Catalog Number
A02049-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RuvB-like 1 RUVBL1 Antibody catalog # A02049-1. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RuvB-like 1 RUVBL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02049-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human RUVBL1/TIP49A/PONTIN(NP_003698.1).
Reactive Species
A02049-1 is reactive to RUVBL1 in Human, Mouse
Calculated molecular weight
50.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02049-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:20-1:50
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using RUVBL1/TIP49A/PONTIN antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded human stomach using RUVBL1/TIP49A/PONTIN Antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded human gastric cancer using RUVBL1/TIP49A/PONTIN Antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using RUVBL1/TIP49A/PONTIN Antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Specific Publications For Anti-RuvB-like 1 RUVBL1 Antibody (A02049-1)
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