Click image to see more details
Product Info Summary
| SKU: | A04067 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SCCA1/2 SERPINB3 Antibody
SKU/Catalog Number
A04067
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SCCA1/2 SERPINB3 Antibody catalog # A04067. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCCA1/2 SERPINB3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04067)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human SERPINB3/4. AA range:111-160
Reactive Species
A04067 is reactive to SERPINB3 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
44.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04067 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of Jurkat, 293 cells using SCCA1/2 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Click image to see more details
Western blot analysis of lysate from Jurkat cells, using SERPINB3/4 Antibody.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:100
Specific Publications For Anti-SCCA1/2 SERPINB3 Antibody (A04067)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SCCA1/2 SERPINB3 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-SCCA1/2 SERPINB3 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-SCCA1/2 SERPINB3 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver cancer using anti-SCCA1/2 antibody A04067. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-07
Question
Is this A04067 anti-SCCA1/2 antibody reactive to the isotypes of SERPINB3?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The immunogen of A04067 anti-SCCA1/2 antibody is Synthesized peptide derived from the Internal region of human SCCA1/2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-03
Question
I see that the anti-SCCA1/2 antibody A04067 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-09
Question
Please see the WB image, lot number and protocol we used for liver cancer using anti-SCCA1/2 antibody A04067. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-03
Question
Do you have a BSA free version of anti-SCCA1/2 antibody A04067 available?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SCCA1/2 antibody A04067 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-17
Question
Would A04067 anti-SCCA1/2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Li
Verified customer
Asked: 2019-05-22
Answer
You can see on the product datasheet, A04067 anti-SCCA1/2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-22
Question
We are currently using anti-SCCA1/2 antibody A04067 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-04
Answer
The anti-SCCA1/2 antibody (A04067) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-04
Question
Will anti-SCCA1/2 antibody A04067 work on goat WB with ectocervix?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
Our lab technicians have not tested anti-SCCA1/2 antibody A04067 on goat. You can run a BLAST between goat and the immunogen sequence of anti-SCCA1/2 antibody A04067 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat ectocervix in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-28
Question
Would anti-SCCA1/2 antibody A04067 work for WB with liver cancer?
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
According to the expression profile of liver cancer, SERPINB3 is highly expressed in liver cancer. So, it is likely that anti-SCCA1/2 antibody A04067 will work for WB with liver cancer.
Boster Scientific Support
Answered: 2017-06-30
Question
I was wanting to use your anti-SCCA1/2 antibody for WB for human liver cancer on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver cancer identification?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
As indicated on the product datasheet, A04067 anti-SCCA1/2 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver cancer in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-26
Question
Is a blocking peptide available for product anti-SCCA1/2 antibody (A04067)?
A. Huang
Verified customer
Asked: 2017-01-23
Answer
We do provide the blocking peptide for product anti-SCCA1/2 antibody (A04067). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-01-23
Question
My question regards to test anti-SCCA1/2 antibody A04067 on human liver cancer for research purposes, then I may be interested in using anti-SCCA1/2 antibody A04067 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Anderson
Verified customer
Asked: 2016-02-23
Answer
The products we sell, including anti-SCCA1/2 antibody A04067, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-02-23
Question
I have a question about product A04067, anti-SCCA1/2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Zhao
Verified customer
Asked: 2013-12-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04067 anti-SCCA1/2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-12-03
