Anti-RANTES/CCL5 Antibody Picoband™

CCL5/RANTES antibody

Boster Bio Anti-RANTES/CCL5 Antibody Picoband™ catalog # PB9573. Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: PB9573
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-RANTES/CCL5 Antibody Picoband™

View all CCL5/RANTES Antibodies

SKU/Catalog Number

PB9573

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RANTES/CCL5 Antibody Picoband™ catalog # PB9573. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RANTES/CCL5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9573)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human RANTES recombinant protein (Position: Y26-S91). Human RANTES shares 83.3% amino acid (aa) sequence identity with both mouse and rat RANTES.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9573 is reactive to CCL5 in Human

Applications

PB9573 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

11 kDa

Calculated molecular weight

9.99kDa

Background of CCL5/RANTES

Chemokine (C-C motif) ligand 5 (also CCL5) is a protein which in humans is encoded by the CCL5 gene. It is also known as RANTES. This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA , 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CCL5 (Source: Uniprot.org, NCBI)

Gene Name

CCL5

Full Name

C-C motif chemokine 5

Weight

9.99kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES CCL5 D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP C-C motif chemokine ligand 5 C-C motif chemokine 5|T-cell specific protein p288|beta-chemokine RANTES|chemokine (C-C motif) ligand 5|eosinophil chemotactic cytokine|regulated upon activation, normally T-expressed, and presumably secreted|small inducible cytokine subfamily A (Cys-Cys), member 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CCL5, check out the CCL5 Infographic

CCL5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9573 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Blockage of C-C Chemokine Ligand 2 Alleviated the Appendicitis-Induced Injury in Rabbit Model

Li Z, Sun Zj, Liao Cg, Ma L, Ma Bf, Zhang Yq. Fertil Steril. 2010 May 15;93(8):2661-7. Doi: 10.1016/J.Fertnstert.2010.01.053. Epub 2010 Mar 2. Regulated Upon Activation Normal T-Cell Expressed And Secreted Originating From The Epididymis Different...

Wei Jh, Feng X, Sun Zj, Cheng P, Ma Bf, Zhao J, Dong Yh, Zhang Yq, Li Z. Reprod Fertil Dev. 2015 Mar 19. Doi: 10.1071/Rd14231. [Epub Ahead Of Print] Different Locations Of Rantes And Its Receptors On Mouse Epididymal Spermatozoa.

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5 Customer Q&As for Anti-RANTES/CCL5 Antibody Picoband™

Question

We have seen staining in human brain. Do you have any suggestions? Is anti-RANTES/CCL5 antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

From what I have seen in literature brain does express CCL5. From what I have seen in Uniprot.org, CCL5 is expressed in blood, leukocyte, brain, among other tissues. Regarding which tissues have CCL5 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 16791620
Brain, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-04-06

Question

Our lab used your anti-RANTES/CCL5 antibody for WB on brain in the past. I am using human, and We are going to use the antibody for ELISA next. We want examining brain as well as leukocyte in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

I have checked the website and datasheets of our anti-RANTES/CCL5 antibody and it seems that PB9573 has been tested on human in both WB and ELISA. Thus PB9573 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-08

Question

My boss were well pleased with the WB result of your anti-RANTES/CCL5 antibody. However we have been able to see positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is CCL5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

From what I have seen in literature, blood does express CCL5. Generally CCL5 expresses in secreted. Regarding which tissues have CCL5 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 16791620
Brain, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-09-23

Question

We are currently using anti-RANTES/CCL5 antibody PB9573 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-02

Answer

The anti-RANTES/CCL5 antibody (PB9573) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-02

Question

I would like using your anti-RANTES/CCL5 antibody for lymphocyte chemotaxis studies. Has this antibody been tested with western blotting on hut whole cell lysate? We would like to see some validation images before ordering.

G. Evans

Verified customer

Asked: 2013-05-27

Answer

We appreciate your inquiry. This PB9573 anti-RANTES/CCL5 antibody is tested on hut whole cell lysate. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-05-27

Order DetailsPrice
PB9573

100μg

$370
PB9573-10ug

10μg sample (liquid)

$99
PB9573-Biotin

100 μg Biotin conjugated

$570
PB9573-Cy3

100 μg Cy3 conjugated

$570
PB9573-Dylight488

100 μg Dylight488 conjugated

$570
PB9573-Dylight550

100 μg Dylight550 conjugated

$570
PB9573-Dylight594

100 μg Dylight594 conjugated

$570
PB9573-FITC

100 μg FITC conjugated

$570
PB9573-HRP

100 μg HRP conjugated

$570
PB9573-APC

100 μg APC conjugated

$670
PB9573-PE

100 μg PE conjugated

$670
PB9573-carrier-free

Carrier Free

$370

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