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Product Info Summary
| SKU: | M05489 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Sec8 (EXOC4) Mouse Monoclonal Antibody [Clone ID: OTI7G9]
SKU/Catalog Number
M05489
Size
100 µl
Description
Boster Bio EXOC4 mouse monoclonal antibody,clone OTI7G9. Catalog# M05489. Tested in WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Sec8 (EXOC4) Mouse Monoclonal Antibody [Clone ID: OTI7G9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05489)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI7G9
Isotype
IgG2b
Immunogen
Full length human recombiant protein of human EXOC4 (NP_001032203) produced in E.coli.
Reactive Species
M05489 is reactive to EXOC4 in Human, Mouse, Rat
Calculated molecular weight
110.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05489 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EXOC4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EXOC4 (1:2000).
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Western blot analysis of extracts (35ug) from 293T cell line by using anti-EXOC4 monoclonal antibody (1:500).
Specific Publications For Anti-Sec8 (EXOC4) Mouse Monoclonal Antibody [Clone ID: OTI7G9] (M05489)
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Customer Q&As
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5 Customer Q&As for Anti-Sec8 (EXOC4) Mouse Monoclonal Antibody [Clone ID: OTI7G9]
Question
Is this M05489 anti-RSec8 antibody (Monoclonal, 14G1) reactive to the isotypes of EXOC4?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The immunogen of M05489 anti-RSec8 antibody (Monoclonal, 14G1) is Recombinant rat rSec8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-10
Question
I was wanting to use your anti-RSec8 antibody (Monoclonal, 14G1) for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2018-08-29
Answer
You can see on the product datasheet, M05489 anti-RSec8 antibody (Monoclonal, 14G1) has been tested for IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-29
Question
Will anti-RSec8 antibody (Monoclonal, 14G1) M05489 work for WB with leukemic t-cell?
M. Anderson
Verified customer
Asked: 2018-06-04
Answer
According to the expression profile of leukemic t-cell, EXOC4 is highly expressed in leukemic t-cell. So, it is likely that anti-RSec8 antibody (Monoclonal, 14G1) M05489 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2018-06-04
Question
I have a question about product M05489, anti-RSec8 antibody (Monoclonal, 14G1). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Anderson
Verified customer
Asked: 2016-07-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05489 anti-RSec8 antibody (Monoclonal, 14G1), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-07-05
Question
We are currently using anti-RSec8 antibody (Monoclonal, 14G1) M05489 for rat tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
V. Johnson
Verified customer
Asked: 2016-04-27
Answer
The anti-RSec8 antibody (Monoclonal, 14G1) (M05489) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-27
