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Product Info Summary
| SKU: | M02329-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-SENP2 Mouse Monoclonal Antibody [Clone ID: OTI1F7]
SKU/Catalog Number
M02329-1
Size
100 µl
Description
Boster Bio SENP2 mouse monoclonal antibody, clone OTI1F7 (formerly 1F7). Catalog# M02329-1. Tested in FC, IF, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-SENP2 Mouse Monoclonal Antibody [Clone ID: OTI1F7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02329-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1F7
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 139-523 of human SENP2 (NP_067640) produced in E.coli.
Reactive Species
M02329-1 is reactive to SENP2 in Dog, Human, Monkey, Mouse, Rat
Calculated molecular weight
67.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02329-1 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SENP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SENP2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SENP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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HEK293T cells transfected with either SENP2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SENP2 antibody (M02329-1)
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Anti-SENP2 mouse monoclonal antibody (M02329-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SENP2.
Specific Publications For Anti-SENP2 Mouse Monoclonal Antibody [Clone ID: OTI1F7] (M02329-1)
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