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Product Info Summary
| SKU: | A04530 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SIP1 GEMIN2 Antibody
SKU/Catalog Number
A04530
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SIP1 GEMIN2 Antibody (Catalog # A04530). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SIP1 GEMIN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04530)
Host
Rabbit
Contents
SIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SIP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human SIP1. The immunogen is located within the last 50 amino acids of SIP1.
Reactive Species
A04530 is reactive to GEMIN2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
31.6 kDa
Background of GEMIN2
SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04530 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SIP1 antibody can be used for detection of SIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SIP1 in HeLa cell lysate with SIP1 antibody at (A) 0.5 and (B) 1μg/mL.
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Immunocytochemistry of SIP1 in HeLa cells with SIP1 antibody at 4 μg/mL.
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Immunofluorescence of SIP1 in HeLa cells with SIP1 antibody at 20 μg/mL.
Specific Publications For Anti-SIP1 GEMIN2 Antibody (A04530)
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