Anti-SIRT1 Antibody Picoband™

Sirtuin 1/SIRT1 antibody

Boster Bio Anti-SIRT1 Antibody Picoband™ catalog # PB9159. Tested in IF, ICC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PB9159
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IF, ICC, WB

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Product Name

Anti-SIRT1 Antibody Picoband™

View all Sirtuin 1/SIRT1 Antibodies

SKU/Catalog Number

PB9159

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SIRT1 Antibody Picoband™ catalog # PB9159. Tested in IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SIRT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9159)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SIRT1 recombinant protein (Position: R112-K311). Human SIRT1 shares 90% amino acid (aa) sequence identity with mouse SIRT1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9159 is reactive to SIRT1 in Human

Applications

PB9159 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

81.681kDa

Background of Sirtuin 1/SIRT1

Sirtuin 1, also known as SIR2L1 or SIRT1, is a protein that in humans is encoded by the SIRT1 gene. It is mapped to 10q21.3. Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, Sirtuin 1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For SIRT1 (Source: Uniprot.org, NCBI)

Gene Name

SIRT1

Full Name

NAD-dependent protein deacetylase sirtuin-1

Weight

81.681kDa

Superfamily

sirtuin family

Alternative Names

EC 3.5.1; S. cerevisiae, homolog) 1; SIR2alpha; SIR2L1; SIR2-like protein 1; SIRT1; Sirtuin 1 SIRT1 SIR2, SIR2L1, SIR2alpha sirtuin 1 NAD-dependent protein deacetylase sirtuin-1|NAD-dependent protein deacylase sirtuin-1|SIR2-like protein 1|regulatory protein SIR2 homolog 1|sirtuin type 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SIRT1, check out the SIRT1 Infographic

SIRT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SIRT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9159 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hou S, Zheng F, Li Y, Gao L, Zhang J. Int J Mol Sci. 2014 Aug 26;15(9):15026-43. Doi: 10.3390/Ijms150915026. The Protective Effect Of Glycyrrhizic Acid On Renal Tubular Epithelial Cell Injury Induced By High Glucose.

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4 Customer Q&As for Anti-SIRT1 Antibody Picoband™

Question

We want using your anti-SIRT1 antibody for negative regulation of protein acetylation studies. Has this antibody been tested with western blotting on sw620 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

I appreciate your inquiry. This PB9159 anti-SIRT1 antibody is validated on hepg2 whole cell lysate, mcf whole cell lysate, sw620 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-11

Question

We have seen staining in human prostate. What should we do? Is anti-SIRT1 antibody supposed to stain prostate positively?

P. Lewis

Verified customer

Asked: 2019-06-21

Answer

Based on literature prostate does express SIRT1. Based on Uniprot.org, SIRT1 is expressed in right adrenal gland, testis, prostate, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378

Boster Scientific Support

Answered: 2019-06-21

Question

We are currently using anti-SIRT1 antibody PB9159 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

M. Baker

Verified customer

Asked: 2017-06-06

Answer

The anti-SIRT1 antibody (PB9159) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-06

Question

My team were satisfied with the WB result of your anti-SIRT1 antibody. However we have been able to see positive staining in right adrenal gland pml body using this antibody. Is that expected? Could you tell me where is SIRT1 supposed to be expressed?

R. Jackson

Verified customer

Asked: 2013-11-14

Answer

From what I have seen in literature, right adrenal gland does express SIRT1. Generally SIRT1 expresses in nucleus, pml body, sirtt1 75 kda fragment: cytoplasm. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378

Boster Scientific Support

Answered: 2013-11-14

Order DetailsPrice
PB9159

100μg

$370
PB9159-10ug

10μg sample (liquid)

$99
PB9159-Biotin

100 μg Biotin conjugated

$570
PB9159-Cy3

100 μg Cy3 conjugated

$570
PB9159-Dylight488

100 μg Dylight488 conjugated

$570
PB9159-Dylight550

100 μg Dylight550 conjugated

$570
PB9159-Dylight594

100 μg Dylight594 conjugated

$570
PB9159-FITC

100 μg FITC conjugated

$570
PB9159-HRP

100 μg HRP conjugated

$570
PB9159-APC

100 μg APC conjugated

$670
PB9159-PE

100 μg PE conjugated

$670
PB9159-carrier-free

Carrier Free

$370

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PB9159
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$370.00

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