Anti-SIT Purified SIT1 Monoclonal Antibody

SIT1 antibody

Boster Bio Anti-SIT Purified SIT1 Monoclonal Antibody (Catalog# M05696). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.

Product Info Summary

SKU: M05696
Size: 0.1 mg
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IP, WB

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Product Name

Anti-SIT Purified SIT1 Monoclonal Antibody

View all SIT1 Antibodies

SKU/Catalog Number

M05696

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SIT Purified SIT1 Monoclonal Antibody (Catalog# M05696). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-SIT Purified SIT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05696)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

SIT-01

Isotype

Mouse IgG1

Immunogen

Bacterially produced recombinant intracellular fragment of human SIT. The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody weakly cross-reacts with murine SIT.

Reactive Species

M05696 is reactive to SIT1 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

21126 MW

Background of SIT1

SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05696 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-5 μg/ml, intracellular staining.
Western blotting: 1-2 μg/ml, reducing conditions.

Validation Images & Assay Conditions

Gene/Protein Information For SIT1 (Source: Uniprot.org, NCBI)

Gene Name

SIT1

Full Name

Signaling threshold-regulating transmembrane adapter 1

Weight

21126 MW

Alternative Names

SIT1 SIT1 SIT, SIT-R signaling threshold regulating transmembrane adaptor 1 signaling threshold-regulating transmembrane adapter 1|SHP-2 interacting transmembrane adaptor protein|SHP2 interacting transmembrane adaptor|SHP2-interacting transmembrane adapter protein|SHP2-interacting transmembrane adaptor protein|gp30/40|suppression inducing transmembrane adaptor 1|suppression-inducing transmembrane adapter 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SIT1, check out the SIT1 Infographic

SIT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SIT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M05696

0.1 mg

$315

Free Secondary Antibody

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M05696
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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