Product Info Summary
SKU: | M05696 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-SIT Purified SIT1 Monoclonal Antibody
SKU/Catalog Number
M05696
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SIT Purified SIT1 Monoclonal Antibody (Catalog# M05696). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-SIT Purified SIT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05696)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SIT-01
Isotype
Mouse IgG1
Immunogen
Bacterially produced recombinant intracellular fragment of human SIT. The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody weakly cross-reacts with murine SIT.
Reactive Species
M05696 is reactive to SIT1 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
21126 MW
Background of SIT1
SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05696 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-5 μg/ml, intracellular staining.
Western blotting: 1-2 μg/ml, reducing conditions.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-SIT Purified SIT1 Monoclonal Antibody M05696
Western blotting of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sampleProtein Target Info & Infographic
Gene/Protein Information For SIT1 (Source: Uniprot.org, NCBI)
Gene Name
SIT1
Full Name
Signaling threshold-regulating transmembrane adapter 1
Weight
21126 MW
Alternative Names
SIT1 SIT1 SIT, SIT-R signaling threshold regulating transmembrane adaptor 1 signaling threshold-regulating transmembrane adapter 1|SHP-2 interacting transmembrane adaptor protein|SHP2 interacting transmembrane adaptor|SHP2-interacting transmembrane adapter protein|SHP2-interacting transmembrane adaptor protein|gp30/40|suppression inducing transmembrane adaptor 1|suppression-inducing transmembrane adapter 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIT1, check out the SIT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIT Purified SIT1 Monoclonal Antibody (M05696)
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