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- Table of Contents
SLC40A1 antibody (A01953-2) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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Flow Cytometry analysis of U20S cells using anti-SLC40A1 antibody (A01953-2).
Overlay histogram showing U20S cells stained with A01953-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC40A1 Antibody (A01953-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U20S cells stained with A01953-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC40A1 Antibody (A01953-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-SLC40A1 Antibody Picoband®
SLC40A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
SLC40A1 antibody was positively detected by WB in:
human placenta tissue, rat liver tissue, mouse spleen tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC40A1 antibody was positively detected by IHC in:
human larynx squamous cell carcinoma tissue, human liver cancer tissue, human ovarian serous adenocarcinoma tissue, human prostate adenocarcinoma tissue, human rectum adenocarcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SLC40A1 antibody was positively detected by IF in:
human ovarian cancer tissue
SLC40A1 antibody was positively detected by FC in:
U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-SLC40A1 Antibody Picoband®
View all SLC40A1 Antibodies
View all SLC40A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SLC40A1 recombinant protein (Position: M1-V571).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
62.5 kDa
Background of SLC40A1
Ferroportin-1, also known as solute carrier family 40 member 1 (SLC40A1) or iron-regulated transporter 1 (IREG1), is a protein that in humans is encoded by the SLC40A1 gene, and is part of the Ferroportin (Fpn) Family.
Cite This Product
Anti-SLC40A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01953-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
92%
High Homology
Rabbit
91%
High Homology
Mouse
91%
High Homology
Sheep
91%
High Homology
Cow
90%
High Homology
Rat
90%
High Homology
Zebrafish
69%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLC40A1 antibody
Immunohistochemistry (IHC) protocol for SLC40A1 antibody
Immunofluorescence (IF) protocol for SLC40A1 antibody
Flow Cytometry (FC) protocol for SLC40A1 antibody
Publications
Application:
Species:
Polygonatum odoratum extract ameliorates acetaminophen-induced acute liver injury by modulating the gut-liver axis and remodeling microbial and ceramide metabolism to suppress hepatic ferroptosis: insights from multi-omics
Published: 2026-07-29
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 42591220
Cyclopeptide heterophyllin from Pseudostellaria heterophylla alleviates neuroinflammation: a study integrating molecular dynamics, inflammatory pathway analysis, and in vivo validation
Published: 2026-06-27
Journal: FOOD SCIENCE AND BIOTECHNOLOGY
Application: WB
Impact Factor: 3.90
Species: Mouse
Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells
Published: 2025-11-29
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.50
Species: Mouse
PMID: 41325790
Astragalus polysaccharide hinders cervical cancer immune escape by targeting NR3C2 and activating SLC40A1
Published: 2025-11-24
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Application: WB,IHC
Impact Factor: 4.20
Species: Human,Mouse
PMID: 41297642
p53 mediates iron overload and ferroptosis via transcriptional regulation of TfR1 in PM2.5-exposed cardiomyocytes
Published: 2025-10-23
Journal: JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
Application: WB
Impact Factor: 4.70
Species: Mouse,Rat
PMID: 41138936
Nuclear factor E2 related factor 2 may alleviate liver injury by regulating iron overload and lipid peroxidation induced by chronic fluorosis
Published: 2025-10-08
Journal: JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
Application: WB
Impact Factor: 3.30
Species: Rat,Human
PMID: 41075730
Muscone attenuates myocardial infarction by regulating ferroptosis of cardiomyocytes through Nrf2/system Xc-/GPX4 signaling pathway
Published: 2025-08-05
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Rat
PMID: 40752108
Ellagic acid alleviates iron overload-induced liver damage by mitigating ferroptosis through modulation of the TGFβ/Smad signaling pathway
Published: 2025-05-09
Journal: FOOD RESEARCH INTERNATIONAL
Application: WB
Impact Factor: 8.00
Species: Mouse
PMID: 40467263
Sulforaphane inhibits multiple myeloma cell-induced osteoclast differentiation and macrophage proliferation by elevating ferroportin1
Published: 2024-12-11
Journal: CANCER CHEMOTHERAPY AND PHARMACOLOGY
Application: WB
Impact Factor: 2.70
Species: Mouse
PMID: 39661165
Exploring the mechanism of Ling-Gui-Zhu-Gan decoction in metabolic cardiomyopathy via inhibiting ferroptosis
Published: 2024-11-09
Journal: PHYTOMEDICINE
Application: WB,IHC
Impact Factor: 6.70
Species: Rat
PMID: 39547098
Ferroptosis is involved in focal segmental glomerulosclerosis in rats
Published: 2023-12-14
Journal: Scientific Reports
Application: WB
Impact Factor: 4.60
Species: Rat
PMID: 38097813
Review
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1 Review For Anti-SLC40A1 Antibody Picoband®
The SLC40A1 antibody was used to detect the expression of the target protein in human uterine tissue. Although two bands were observed, the differences in expression levels were still clearly discernible.
| SKU | A01953-2 |
|---|---|
| Application | Western Blot |
| Sample | human uterine tissue |
| Sample Processing Description | The tissue was minced and further disrupted by sonication, then lysed on ice for 1 hour using RIPA buffer. After centrifugation, the supernatant was collected and quantified using the BCA method. The appropriate amount of loading buffer was added, and the samples were boiled in a water bath to denature the proteins. Finally, 15 μL of each protein sample was loaded into each lane of the SDS-PAGE gel. |
| Other Reagents | 5% Non-fat milk |
| Primary Antibody | Anti-SLC40A1 Antibody Picoband® |
| Primary Incubation | overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon |
| Results Summary | The SLC40A1 antibody was used to detect the expression of the target protein in human uterine tissue. Although two bands were detected, the differences in expression levels were still clearly observable and did not affect the analysis of the experimental results. |
Anfeng Ning, Peking University Third Hospital
Verified customer
Submitted 2025-11-11
Product Promise
We promise Anti-SLC40A1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01953-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026