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Product Info Summary
| SKU: | M04880 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Pig |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-SLP76 Purified LCP2 Monoclonal Antibody
SKU/Catalog Number
M04880
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SLP76 Purified LCP2 Monoclonal Antibody (Catalog# M04880). Tested in WB, IHC-P application(s). This antibody reacts with Pig, Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-SLP76 Purified LCP2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04880)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SLP-76/03
Isotype
Mouse IgG2b
Immunogen
Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag. The monoclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M04880 is reactive to LCP2 in Human, Mouse, Pig
Observed Molecular Weight
42 kDa
Calculated molecular weight
60.2 kDa
Background of LCP2
SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04880 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: thymus
Validation Images & Assay Conditions
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Western blotting analysis (reducing conditions) of whole cell lysate using anti-SLP76 (SLP-76/03).
Lane 1: HPB-ALL human peripheral blood T cell leukemia cell line
Lane 2: JURKAT human peripheral blood T cell leukemia cell line
Lane 3: RAMOS human Burkitt lymphoma cell line
Specific Publications For Anti-SLP76 Purified LCP2 Monoclonal Antibody (M04880)
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