Anti-DHFR Antibody Picoband™ (monoclonal, 3C8)

Boster Bio Anti-DHFR Antibody Picoband™ (monoclonal, 3C8) catalog # M00813-1. Tested in IHC-P, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: M00813-1
Size: 100μg/vial
Reactive Species: Human, Rat
Host: Mouse
Application: IHC-P, WB

Product Name

Anti-DHFR Antibody Picoband™ (monoclonal, 3C8)

See all Dihydrofolate Reductase/DHFR products

SKU/Catalog Number

M00813-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DHFR Antibody Picoband™ (monoclonal, 3C8) catalog # M00813-1. Tested in IHC-P, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DHFR Antibody Picoband™ (monoclonal, 3C8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00813-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

3C8

Isotype

IgG2b

Immunogen

E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR?shares?90%?amino?acid? (aa)?sequence?identity?with both mouse?and?rat DHFR.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M00813-1 is reactive to DHFR in Human, Rat

Applications

M00813-1 is guaranteed for IHC-P, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For DHFR (Source: Uniprot.Org, NCBI)

Uniprot ID

P00374

Gene ID

1719

Gene Name

DHFR

Full Name

Dihydrofolate reductase

Weight

21.453kDa

Superfamily

dihydrofolate reductase family

Alternative Names

DHFR; DHFRP1; Dihydrofolate Reductase; DYR; EC 1.5.1.3 DHFR DHFRP1, DYR dihydrofolate reductase dihydrofolate reductase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DHFR, check out the DHFR Infographic

DHFR infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DHFR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-DHFR Antibody Picoband™ (monoclonal, 3C8)

Question

We are currently using anti-DHFR antibody (monoclonal, 3C8) M00813-1 for rat tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

The anti-DHFR antibody (monoclonal, 3C8) (M00813-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-04

Question

We ordered your anti-DHFR antibody (monoclonal, 3C8) for WB on eye in a previous experiment. I am using rat, and We intend to use the antibody for IHC-P next. We need examining eye as well as buccal mucosa cell in our next experiment. Could give a recommendation on which antibody would work the best for IHC-P?

E. Williams

Verified customer

Asked: 2018-10-03

Answer

I looked at the website and datasheets of our anti-DHFR antibody (monoclonal, 3C8) and I see that M00813-1 has been validated on rat in both WB and IHC-P. Thus M00813-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-03

Question

My colleagues were satisfied with the WB result of your anti-DHFR antibody (monoclonal, 3C8). However we have been able to see positive staining in buccal mucosa cell mitochondrion using this antibody. Is that expected? Could you tell me where is DHFR supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-06-26

Answer

According to literature, buccal mucosa cell does express DHFR. Generally DHFR expresses in mitochondrion. Regarding which tissues have DHFR expression, here are a few articles citing expression in various tissues:
Eye, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-06-26

Question

We have been able to see staining in rat buccal mucosa cell. Are there any suggestions? Is anti-DHFR antibody (monoclonal, 3C8) supposed to stain buccal mucosa cell positively?

A. Krishna

Verified customer

Asked: 2017-10-31

Answer

According to literature buccal mucosa cell does express DHFR. According to Uniprot.org, DHFR is expressed in buccal mucosa cell, eye, among other tissues. Regarding which tissues have DHFR expression, here are a few articles citing expression in various tissues:
Eye, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-10-31

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M00813-1
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