Anti-SLUG/SNAI2 Antibody Picoband®

IHC analysis of SNAI2 using anti-SNAI2 antibody (PB9439).
SNAI2 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAI2 Antibody (PB9439) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

EMP3 affects the malignant phenotype of glioblastoma by promoting the EMT process ( A )-( D ). Correlation analysis of VIM , FOS , SNAI2 , TWIST1 and EMP3 using TCGA data ( E )-( H ). These related EMT markers were tested by qRT-PCR after the knockdown of EMP3 for 96 h cultured with siRNA. On the other hand, proteins of EMT markers VIM, SNAI2, FOS, TWIST1 were investigated by western blot after EMP3 siRNA transient transfection ( I )-( M ), Actin as the internal parameter. Cells were divided into three groups, NC (negtive control) group, siRNA EMP3-1 and siRNA EMP3-2 group. U87 cells were lysis after siRNA incubation for 72 h. The efficiency of trasfection of siRNA EMP3 was detected by WB ( N ). All of the full-length blots/gels of western blot are presented in Supplementary Figure C-H. * p < 0.05, ** P < 0.01, *** P < 0.001,**** p < 0.0001
Index in PubMed under a CC BY license. PMID: 38229014

Western blot analysis of SNAI2 using anti-SNAI2 antibody (PB9439).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI2 antigen affinity purified polyclonal antibody (Catalog # PB9439) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI2 at approximately 30 kDa. The expected band size for SNAI2 is at 30 kDa.

IHC analysis of SNAI2 using anti-SNAI2 antibody (PB9439).
SNAI2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAI2 Antibody (PB9439) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.