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Product Info Summary
| SKU: | A00884-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Glucokinase/GCK Antibody Picoband®
SKU/Catalog Number
A00884-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glucokinase/GCK Antibody Picoband® catalog # A00884-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucokinase/GCK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00884-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Glucokinase recombinant protein (Position: Y234-T431).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00884-1 is reactive to GCK in Human, Mouse, Rat
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.2 kDa
Background of GCK
Glucokinase (GCK) is an enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00884-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human Jurkat whole cell, rat liver tissue, rat RH35 whole cell, mouse HEPA6/1 whole cell
ICC/IF: SiHa cell
Validation Images & Assay Conditions
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Western blot analysis of Glucokinase using anti-Glucokinase antibody (A00884-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse HEPA6/1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glucokinase antigen affinity purified polyclonal antibody (A00884-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Glucokinase at approximately 52 kDa. The expected band size for Glucokinase is at 52 kDa.
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IF analysis of Glucokinase using anti-Glucokinase antibody (A00884-1).
Glucokinase was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Glucokinase Antibody (A00884-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Glucokinase/GCK Antibody Picoband® (A00884-1)
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Customer Q&As
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7 Customer Q&As for Anti-Glucokinase/GCK Antibody Picoband®
Question
Is this A00884-1 anti-Glucokinase/GCK antibody reactive to the isotypes of GCK?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The immunogen of A00884-1 anti-Glucokinase/GCK antibody is E. coli-derived human Glucokinase recombinant protein (Position: Y234-T431). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-23
Question
My question regarding product A00884-1, anti-Glucokinase/GCK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Rodriguez
Verified customer
Asked: 2019-12-10
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00884-1 anti-Glucokinase/GCK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-10
Question
We are currently using anti-Glucokinase/GCK antibody A00884-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
R. Wu
Verified customer
Asked: 2018-08-17
Answer
The anti-Glucokinase/GCK antibody (A00884-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-17
Question
Here is the WB image, lot number and protocol we used for liver using anti-Glucokinase/GCK antibody A00884-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-06
Question
Will A00884-1 anti-Glucokinase/GCK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Li
Verified customer
Asked: 2016-10-06
Answer
You can see on the product datasheet, A00884-1 anti-Glucokinase/GCK antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-10-06
Question
My question regards to test anti-Glucokinase/GCK antibody A00884-1 on rat liver for research purposes, then I may be interested in using anti-Glucokinase/GCK antibody A00884-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Miller
Verified customer
Asked: 2015-06-16
Answer
The products we sell, including anti-Glucokinase/GCK antibody A00884-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-06-16
Question
I see that the anti-Glucokinase/GCK antibody A00884-1 works with ELISA, what is the protocol used to produce the result images on the product page?
J. Anderson
Verified customer
Asked: 2013-12-27
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-12-27
