Click image to see more details
Product Info Summary
| SKU: | A00728-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SMAD1 Antibody
SKU/Catalog Number
A00728-2
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-SMAD1 Antibody (Catalog # A00728-2). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-SMAD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00728-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein.
Reactive Species
A00728-2 is reactive to SMAD1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
52.3 kDa
Background of SMAD1
SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro; this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00728-2 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:25,000 - 1:30,000
IP: 2 ug
WB: 1:500
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to phosphorylated SMAD1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. This antibody is specific for the phosphorylated pS206 of SMAD1. Stimulation of EGF, TGFbeta BMP2, and 0.5M NaCl are recommended for 1 hour. This antibody is useful in ChIP and ChIPSeq.
Validation Images & Assay Conditions
Click image to see more details
Western blot using Boster's Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 µg of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX
Specific Publications For Anti-SMAD1 Antibody (A00728-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SMAD1 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-SMAD1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-SMAD1 Antibody
Question
See below the WB image, lot number and protocol we used for kidney using anti-SMAD1 antibody A00728-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-06
Question
I was wanting to use your anti-SMAD1 antibody for WB for rat kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat kidney identification?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
As indicated on the product datasheet, A00728-2 anti-SMAD1 antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-24
Question
My team were happy with the WB result of your anti-SMAD1 antibody. However we have seen positive staining in visceral pleura cytoplasm. using this antibody. Is that expected? Could you tell me where is SMAD1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
According to literature, visceral pleura does express SMAD1. Generally SMAD1 expresses in cytoplasm. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2020-03-03
Question
Does A00728-2 anti-SMAD1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
You can see on the product datasheet, A00728-2 anti-SMAD1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-29
Question
We have been able to see staining in rat placenta. Do you have any suggestions? Is anti-SMAD1 antibody supposed to stain placenta positively?
D. Li
Verified customer
Asked: 2019-09-19
Answer
According to literature placenta does express SMAD1. According to Uniprot.org, SMAD1 is expressed in visceral pleura, kidney, mammary carcinoma, placenta, uterus, ovarian carcinoma, among other tissues. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2019-09-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-SMAD1 antibody A00728-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-26
Question
Can you help my question with product A00728-2, anti-SMAD1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00728-2 anti-SMAD1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-07
Question
Is a blocking peptide available for product anti-SMAD1 antibody (A00728-2)?
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
We do provide the blocking peptide for product anti-SMAD1 antibody (A00728-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-10-17
Question
Would anti-SMAD1 antibody A00728-2 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
According to the expression profile of kidney, SMAD1 is highly expressed in kidney. So, it is likely that anti-SMAD1 antibody A00728-2 will work for WB with kidney.
Boster Scientific Support
Answered: 2018-10-17
Question
Is this A00728-2 anti-SMAD1 antibody reactive to the isotypes of SMAD1?
C. Li
Verified customer
Asked: 2018-06-19
Answer
The immunogen of A00728-2 anti-SMAD1 antibody is This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-19
Question
I see that the anti-SMAD1 antibody A00728-2 works with WB, what is the protocol used to produce the result images on the product page?
J. Jones
Verified customer
Asked: 2017-09-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-09-05
Question
We are currently using anti-SMAD1 antibody A00728-2 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
The anti-SMAD1 antibody (A00728-2) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-21
Question
Is there a BSA free version of anti-SMAD1 antibody A00728-2 available?
M. Jackson
Verified customer
Asked: 2015-12-07
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SMAD1 antibody A00728-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-12-07
Question
We are interested in to test anti-SMAD1 antibody A00728-2 on rat kidney for research purposes, then I may be interested in using anti-SMAD1 antibody A00728-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Rodriguez
Verified customer
Asked: 2014-02-26
Answer
The products we sell, including anti-SMAD1 antibody A00728-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-02-26
Question
We ordered your anti-SMAD1 antibody for WB on uterus a few months ago. I am using human, and I plan to use the antibody for IP next. I was wanting to use examining uterus as well as kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
D. Parker
Verified customer
Asked: 2013-10-10
Answer
I viewed the website and datasheets of our anti-SMAD1 antibody and it seems that A00728-2 has been validated on human in both WB and IP. Thus A00728-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-10-10
