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- Table of Contents
Smad1 antibody (PA2114) performs well in IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Anti-Smad1 antibody, PA2114, ICC
ICC: A549 Cell
ICC: A549 Cell
Designing a Western blot or IHC for SMAD1? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-Smad1 Antibody Picoband®
Smad1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, ICC, WB |
Tested Applications
Smad1 antibody was positively detected by WB in:
SMMC Cell, K562 Cell, HT1080 Cell, HELA Cell, JURKAT Cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Smad1 antibody was positively detected by IHC in:
Human Lung Cancer Tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Smad1 antibody was positively detected by ICC in:
A549 Cell
Recommend Dilution
Application
Dilution
Species
Immunocytochemistry
0.5-1μg/ml
Human, -
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat, Mouse
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Human
Tested
Recommend
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Product Information
Product Name
Anti-Smad1 Antibody Picoband®
View all SMAD1 Antibodies
View all SMAD1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Smad1, different from the related rat sequence by one amino acid, and different from the related human sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated Molecular Weight
52.2 kDa
Background of Smad1
Mothers against decapentaplegic homolog 1 also known as SMAD family member 1 or SMAD1 is a protein that in humans is encoded by the SMAD1 gene. SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene "mothers against decapentaplegic" (Mad) and the C. elegans gene Sma. The gene was assigned to human chromosome 4q31.21. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
Cite This Product
Anti-Smad1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2114)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
93%
High Homology
Human
87%
Moderate Homology
Cow
87%
Moderate Homology
Sheep
87%
Moderate Homology
Dog
87%
Moderate Homology
Monkey
87%
Moderate Homology
Rabbit
67%
Low homology
Zebrafish
67%
Low homology
Drosophila
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Smad1 antibody
Immunohistochemistry (IHC) protocol for Smad1 antibody
Immunocytochemistry (ICC) protocol for Smad1 antibody
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Product Promise
We promise Anti-Smad1 Antibody Picoband® performs excellently in relevant applications for IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2114
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 26, 2026